纯度 | >85%SDS-PAGE. |
种属 | Human |
靶点 | TPSAB1 |
Uniprot No | Q15661 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 31-275aa |
氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSHMIVGGQEAPRSKWPWQVSLRVHGPYW MHFCGGSLIHPQWVLTAAHCVGPDVKDLAALRVQLREQHLYYQDQLLPVS RIIVHPQFYTAQIGADIALLELEEPVNVSSHVHTVTLPPASETFPPGMPC WVTGWGDVDNDERLPPPFPLKQVKVPIMENHICDAKYHLGAYTGDDVRIV RDDMLCAGNTRRDSCQGDSGGPLVCKVNGTWLQAGVVSWGEGCAQPNRPG IYTRVTYYLDWIHHYVPKKP |
预测分子量 | 30 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇示例性参考文献(请注意,部分内容为示例性概括,非真实文献):
1. **"Recombinant human tryptase beta-1: expression, purification, and enzymatic characterization"**
- 作者:Caughey GH, et al.
- 摘要:研究通过昆虫细胞表达系统成功制备重组TPSAB1蛋白,分析了其酶活性及抑制剂敏感性,证实其与天然类胰蛋白酶相似的底物特异性。
2. **"Structural insights into TPSAB1 activation and substrate recognition by X-ray crystallography"**
- 作者:Akin C, et al.
- 摘要:解析了重组TPSAB1蛋白的晶体结构,揭示了其四聚体构象及活性位点关键氨基酸残基,为开发靶向抑制剂提供结构基础。
3. **"Role of recombinant TPSAB1 in mast cell-mediated allergic inflammation in vitro"**
- 作者:Metz M, et al.
- 摘要:利用重组TPSAB1蛋白模拟肥大细胞活化,证明其诱导上皮细胞炎性因子释放,并验证其在哮喘模型中促进气道高反应性的作用机制。
4. **"Optimization of TPSAB1 recombinant protein production in mammalian expression systems"**
- 作者:Fajardo I, et al.
- 摘要:对比哺乳动物与大肠杆菌表达系统,优化重组TPSAB1的分泌表达和纯化流程,获得高纯度、高活性的蛋白用于功能研究。
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**说明**:以上文献标题及摘要为示例性质,实际研究中需根据具体论文调整内容。建议通过PubMed或Web of Science以“TPSAB1 recombinant protein”为关键词检索真实文献。
TPSAB1 (Tryptase Alpha/Beta 1) is a gene encoding a member of the tryptase serine protease family, primarily expressed in mast cells. Mast cell tryptases, including the TPSAB1-derived β1 isoform, play critical roles in immune responses, particularly in allergic reactions, asthma, and host defense. These proteases are stored in mast cell granules and released upon activation, contributing to extracellular matrix degradation, inflammation, and tissue remodeling through cleavage of substrates like fibrinogen, cytokines, and protease-activated receptors (PARs). TPSAB1 has garnered attention due to its involvement in mast cell-mediated pathologies and potential as a therapeutic target.
Recombinant TPSAB1 protein is produced using biotechnological systems (e.g., mammalian or insect cell lines) to ensure proper post-translational modifications, including tetramer formation essential for enzymatic activity. This engineered protein serves as a vital tool for studying tryptase functions, inhibitor screening, and elucidating mechanisms in mast cell disorders. For example, it enables researchers to analyze substrate specificity, evaluate drug candidates for allergic diseases, and investigate genetic variants linked to conditions like mast cell activation syndrome (MCAS), where TPSAB1 duplication or overexpression is implicated.
Additionally, recombinant TPSAB1 aids in structural studies, providing insights into active-site conformations to guide rational drug design. Its applications extend to biomarker research, as elevated tryptase levels in blood correlate with systemic mastocytosis and anaphylaxis severity. Despite challenges in maintaining stability during production, recombinant TPSAB1 remains indispensable for advancing mast cell biology and developing targeted therapies against tryptase-driven diseases.
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