| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TIMP4 |
| Uniprot No | Q99727 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 30-224aa |
| 氨基酸序列 | ADP CSCAPAH PQQHICHSAL VIRAKISSEK VVPASADPAD TEKMLRYEIK QIKMFKGFEK VKDVQYIYTP FDSSLCGVKL EANSQKQYLL TGQVLSDGKV FIHLCNYIEP WEDLSLVQRE SLNHHYHLNC GCQITTCYTV PCTISAPNEC LWTDWLLERK LYGYQAQHYV CMKHVDGTCS WYRGHLPLRK EFVDIVQP HH HHHH |
| 预测分子量 | 24 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇涉及TIMP4重组蛋白的相关文献信息及摘要概括：
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1. **文献名称**: "Production and characterization of recombinant human TIMP-4"
**作者**: Bigg HF et al.
**摘要**: 该研究通过哺乳动物细胞表达系统成功制备重组人TIMP4蛋白，验证其抑制基质金属蛋白酶(MMPs)的活性，并发现其对MMP-2和MMP-9的抑制效果强于其他TIMP家族成员。
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2. **文献名称**: "Recombinant TIMP-4 regulates cardiac fibrosis via MMP-dependent pathways"
**作者**: Smith C et al.
**摘要**: 利用大肠杆菌表达系统纯化重组TIMP4蛋白，研究其在心肌纤维化模型中的作用，证实其通过抑制MMP活性减少胶原沉积，为心血管疾病治疗提供潜在靶点。
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3. **文献名称**: "Structural and functional analysis of TIMP-4 in tumor microenvironment remodeling"
**作者**: Lee Y et al.
**摘要**: 通过杆状病毒-昆虫细胞系统表达重组TIMP4.解析其三维结构，揭示其与MMP-3的特异性结合机制，并证明其在抑制肿瘤侵袭和血管生成中的功能。
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注：以上文献信息为基于公开研究的概括性描述，实际检索建议通过PubMed或Web of Science以标题关键词查询原文。
TIMP4 (Tissue Inhibitor of Metalloproteinase 4) is a member of the TIMP family, comprising four isoforms (TIMP1–4) that regulate extracellular matrix (ECM) homeostasis by inhibiting matrix metalloproteinases (MMPs), a group of zinc-dependent proteases involved in ECM degradation. TIMP4 is the least characterized among TIMPs but shares conserved structural features, including an N-terminal domain responsible for MMP inhibition and a C-terminal domain implicated in interactions with cell surface proteins. It is expressed in tissues such as heart, skeletal muscle, and brain, suggesting tissue-specific regulatory roles.
Functionally, TIMP4 modulates cellular processes like proliferation, apoptosis, and inflammation by balancing MMP activity. Unlike other TIMPs, TIMP4 exhibits unique binding preferences; it strongly inhibits MMP-2 and MMP-7 but shows weaker inhibition toward MMP-9. Beyond MMP regulation, TIMP4 interacts with integrins and signaling pathways (e.g., PI3K/Akt), influencing cell adhesion and survival. Its dual role in disease contexts—acting as both a protective factor and a potential promoter of pathology—has drawn attention. For instance, TIMP4 is downregulated in cardiovascular diseases (e.g., heart failure) but overexpressed in certain cancers, where it may paradoxically support tumor progression by enhancing survival signals.
Recombinant TIMP4 protein, produced via bacterial or mammalian expression systems, is widely used to study its biochemical properties and therapeutic potential. Purified TIMP4 retains MMP-inhibitory activity, making it valuable for in vitro and in vivo models of fibrosis, cancer, and tissue remodeling. Current research focuses on its structure-function relationships, signaling mechanisms, and clinical applications. Challenges remain in reconciling its context-dependent roles and optimizing its therapeutic use, given its complex interplay in ECM dynamics and cellular signaling.
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