首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人头蛋白(NOG)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的NOG含量。试剂盒检测范围为1.56 ng/mL-100 ng/mL，适用于体外定量分析科研场景中NOG的表达变化，例如研究其在骨骼发育、神经分化或相关疾病模型中的调控机制，为分子生物学、细胞信号通路研究及基础医学实验提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Nog ELISA Kit; NOGG_HUMAN ELISA Kit; Noggin ELISA Kit; SYM 1 ELISA Kit; SYM1 ELISA Kit; Symphalangism 1 (proximal) ELISA Kit; Synostoses (multiple) syndrome 1 ELISA Kit; SYNS 1 ELISA Kit; SYNS1 ELISA Kit
缩写：NOG
Uniprot No.：Q13253
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Developmental Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human NOG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of human NOG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 97 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1002.3782.3982.3882.242501.6011.6321.6171.471251.0541.0681.0610.91512.50.6120.6380.6250.4796.250.4270.4470.4370.2913.120.3050.3260.3160.1701.560.2510.2690.2600.11400.1450.1470.146 | ||||||
货期：3-5 working days
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