首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人神经调节肽U(NMU)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、脑脊液（CSF）、组织匀浆样本中的NMU含量。NMU（神经介素 U）是一种神经肽。它在体内多种生理过程中发挥作用，如能量代谢、心血管调节等。其研究机制主要围绕与特定受体结合，激活下游信号通路，影响细胞的功能和机体的生理状态，目前针对它在疾病治疗等方面的研究也在不断深入。试剂盒检测范围为0.312 ng/mL-20 ng/mL，稳定的性能适用于探究NMU在神经系统疾病模型、代谢相关机制或生理调控中的表达变化，例如通过分析不同病理条件下脑脊液或组织样本的NMU水平，揭示其与能量稳态、应激反应的关联性。为体外实验研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Neuromedin U 25 ELISA Kit; Neuromedin U ELISA Kit; Neuromedin-U-25 ELISA Kit; NmU 25 ELISA Kit; NMU ELISA Kit; NmU-25 ELISA Kit; NMU_HUMAN ELISA Kit
缩写：NMU
Uniprot No.：P48645
种属：Homo sapiens (Human)
样本类型：serum, plasma, cerebrospinal fluid (CSF), tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of Human NMU in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 84-98 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 86-98 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 84-98 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 88-98 | |||||
回收率：
| The recovery of Human NMU spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 92-102 | ||||
| EDTA plasma (n=4) | 88 | 84-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.5852.3872.4862.314102.0581.8961.9771.80551.3751.3041.3401.1682.50.8520.8790.8660.6941.250.5740.5520.5630.3910.6250.3690.3840.3770.2050.3120.2710.2650.2680.09600.1720.1710.172 | ||||||
货期：3-5 working days
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