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Human N-acetyl-β-D-glucosaminidase,NAG ELISA Kit

  • 中文名： 人N-乙酰-β-D-氨基葡萄糖苷酶(NAG)ELISA Kit
货号: IPD-E09450h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人N-乙酰-β-D-氨基葡萄糖苷酶(NAG)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、尿液样本中的NAG含量。N-乙酰-β-D-氨基葡萄糖苷酶（NAG）是一种溶酶体水解酶，主要存在于近端肾小管，其活性升高是肾小管损伤的早期标志。研究显示，NAG在肾脏疾病如肾病综合征、肾盂肾炎等中升高，且其活性变化与疾病严重程度相关。通过基因工程和代谢工程，可以优化NAG的合成途径，提高其产量，为相关疾病的治疗提供新的思路。试剂盒检测范围为7.81 mIU/mL-2000 mIU/mL，在肾脏疾病研究、药物毒性评估及生理代谢机制探索中具有重要价值；支持科研人员高效开展肾损伤相关模型构建、疾病机理研究或生物标志物筛选等工作；尤其适用于尿液样本中NAG活性变化的动态监测，为肾脏功能评估提供可靠实验工具；助力基础医学与生命科学领域的实验研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Beta hexosaminidase beta chain ELISA Kit; Beta hexosaminidase subunit beta ELISA Kit; Beta N acetylhexosaminidase ELISA Kit; Beta-hexosaminidase subunit beta chain A ELISA Kit; Beta-N-acetylhexosaminidase subunit beta ELISA Kit; Cervical cancer proto oncogene 7 protein ELISA Kit; Cervical cancer proto-oncogene 7 protein ELISA Kit; ENC 1AS ELISA Kit; Epididymis luminal protein 248 ELISA Kit; HCC 7 ELISA Kit; HCC-7 ELISA Kit; HCC7 ELISA Kit; HEL 248 ELISA Kit; HEX B ELISA Kit; Hexb ELISA Kit; HEXB_HUMAN ELISA Kit; Hexosaminidase B (beta polypeptide) ELISA Kit; Hexosaminidase B ELISA Kit; Hexosaminidase subunit B ELISA Kit; HexosaminidaseB ELISA Kit; N acetyl beta glucosaminidase ELISA Kit; N-acetyl-beta-glucosaminidase subunit beta ELISA Kit
  • 缩写：
    NAG
  • Uniprot No.：
    P07686
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, urine
  • 检测范围：
    7.81 mIU/mL-2000 mIU/mL
  • 灵敏度：
    7.81 mIU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<12%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<15%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human NAG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    95


    Range %

    89-101


    1:2

    Average %

    98


    Range %

    92-106


    1:4

    Average %

    96


    Range %

    88-102


    1:8

    Average %

    90


    Range %

    85-98


  • 回收率：

    The recovery of human NAG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    92

    86-99


    EDTA plasma (n=4)

    89

    84-94


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    mIU/ml

    OD1

    OD2

    Average



    7.81

    2.263

    2.383

    2.323



    31.25

    1.246

    1.259

    1.253



    125

    0.568

    0.532

    0.550



    500

    0.265

    0.282

    0.274



    2000

    0.146

    0.153

    0.150



  • 本试剂盒所含材料：



    • A 96-well Assay plate --The 96-well plate has been pre-coated with NAG.

    • Standard (2 x 250 μl)--Dilute the standard at dilution series, read the OD values, and then draw a standard curve.

    • Anti-human NAG Antibody (100 x concentrate) (1 x 60 μl)

    • Antibody Diluent (1 x 10 ml) --Dilute the anti-human NAG antibody.

    • HRP-conjugated goat-anti-mouse NAG antibody (100 x concentrate) (1 x 120 μl) --Act as the detection antibody.

    • HRP-conjugate Diluent (1 x 20 ml) --dilute the HRP-conjugated NAG antibody.

  • 本试剂盒不含材料：



    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm - 570 nm.

    • An incubator that can provide stable incubation conditions up to 37°C±5°C.

    • Centrifuge

    • Vortex

    • Squirt bottle, manifold dispenser, or automated microplate washer

    • Absorbent paper for blotting the microtiter plate


  • 货期：
    3-5 working days

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