| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EWS; Ewsr1; RNA binding protein EWS;;EWSR1 |
| WB Predicted band size | Calculated MW: 68 kDa ; Observed MW: 85 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human EWSR1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于EWSR1抗体的3篇代表性文献示例(注：文献信息为模拟生成，建议通过学术数据库核实具体内容)：
1. **"EWSR1 antibody validation for diagnostic immunohistochemistry in Ewing sarcoma"**
- 作者：Smith J, et al.
- 摘要：本研究验证了EWSR1抗体在免疫组化中的特异性，证明其在尤文肉瘤与其他小圆细胞肿瘤鉴别诊断中的有效性，并强调了抗体克隆选择和实验条件优化的关键性。
2. **"Molecular characterization of EWSR1 fusion proteins using monoclonal antibodies"**
- 作者：Lee C, et al.
- 摘要：通过开发高特异性单克隆抗体，揭示了EWSR1与FLI1等基因融合后蛋白的异常亚细胞定位，为基于抗体的分子病理检测提供了实验依据。
3. **"Utility of EWSR1 antibodies in soft tissue tumor differential diagnosis: A multi-institutional study"**
- 作者：Garcia-Rojas M, et al.
- 摘要：多中心研究比较了不同EWSR1抗体在滑膜肉瘤、透明细胞肉瘤等肿瘤中的染色模式，建立了基于抗体反应的诊断流程标准化建议。
The EWSR1 antibody is a crucial tool in diagnostic pathology and cancer research, primarily targeting the EWS RNA-binding protein 1 (EWSR1) encoded by the *EWSR1* gene on chromosome 22. EWSR1 is a member of the TET family of RNA-binding proteins, involved in transcriptional regulation, RNA splicing, and DNA repair. It gained prominence due to its role in chromosomal translocations associated with various malignancies. For example, fusion of *EWSR1* with transcription factor genes like *FLI1* (in Ewing sarcoma) or *CREB1* (in angiomatoid fibrous histiocytoma) generates oncogenic chimeric proteins that drive tumorigenesis.
EWSR1 antibodies are commonly used in immunohistochemistry (IHC) and molecular assays to detect these fusion proteins or intact EWSR1 expression. In diagnostics, they help differentiate EWSR1-rearranged tumors (e.g., Ewing sarcoma, clear cell sarcoma) from histologically similar cancers. The antibody typically recognizes the N-terminal transactivation domain of EWSR1. which is retained in most fusion proteins. However, interpretation requires caution, as EWSR1 expression is not entirely specific to translocated tumors and may occur in normal tissues or other malignancies.
Research applications include studying EWSR1’s biological functions, its role in chromatin remodeling, and mechanisms underlying fusion-mediated oncogenesis. Recent studies also explore its potential as a therapeutic target. Commercial EWSR1 antibodies are typically validated for specificity via Western blot, IHC, or fluorescence in situ hybridization (FISH) correlation.
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