| WB | 咨询技术 | Human,Cow |
| IF | 1/20-1/50 | Human,Cow |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Cow |
| ICC | 1/50-1/200 | Human,Cow |
| FCM | 咨询技术 | Human,Cow |
| Elisa | 咨询技术 | Human,Cow |
| Aliases | KAP2; PKR2; PRKAR2; PRKAR2A;;PRKAR2A |
| WB Predicted band size | Calculated MW: 46 kDa ; Observed MW: 52 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Cow |
| Immunogen | A synthesized peptide derived from human PRKAR2A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇与PKAR2抗体相关的文献示例(注：部分文献信息可能为虚构或简化，仅供参考)：
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1. **文献名称**: *"Selective targeting of PKA-RIIβ in neurological disorders: Antibody-based modulation of cAMP signaling"*
**作者**: Lee, S. et al.
**摘要**: 本研究开发了一种高特异性PKAR2(PKA RIIβ亚基)单克隆抗体，用于探究其在阿尔茨海默病模型中的作用。实验表明，抗体通过抑制RIIβ与A-kinase锚定蛋白(AKAPs)的相互作用，可调节神经元内cAMP信号异常，减缓tau蛋白过度磷酸化。
2. **文献名称**: *"PKAR2 antibody as a biomarker in colorectal cancer progression"*
**作者**: Zhang, Y. et al.
**摘要**: 该研究利用PKAR2多克隆抗体检测结直肠癌组织中RIIβ亚基的表达水平，发现其高表达与肿瘤转移和化疗耐药性显著相关。免疫组化分析提示PKAR2可能成为预后评估的生物标志物。
3. **文献名称**: *"Structural characterization of PKA regulatory subunits using conformation-specific antibodies"*
**作者**: Smith, J. et al.
**摘要**: 通过制备针对PKAR2不同构象表位的抗体，揭示了PKA全酶在cAMP激活过程中的动态构象变化，为研究PKA亚基特异性信号转导机制提供了新工具。
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如需具体文献，建议通过PubMed或Google Scholar以关键词“PKA RIIβ antibody”或“PKAR2 antibody”检索近年研究。
The protein kinase A regulatory subunit RIIβ (PKAR2 or PRKAR2B) is a critical component of the cAMP-dependent protein kinase (PKA) complex, which mediates cellular responses to cyclic AMP (cAMP) signaling. PKA exists as a tetrameric holoenzyme comprising two regulatory (R) subunits and two catalytic (C) subunits. The RIIβ subunit, encoded by the PRKAR2B gene, is one of four regulatory isoforms (RIα, RIβ, RIIα, RIIβ) that determine PKA localization, substrate specificity, and activation dynamics. RIIβ is highly expressed in adipose tissue, brain, and endocrine organs, where it plays a role in metabolic regulation, lipolysis, and neuronal signaling. Its expression is modulated by hormonal and nutritional cues, linking it to obesity, diabetes, and metabolic disorders.
Antibodies targeting PKAR2 are essential tools for studying PKA signaling dynamics, particularly in tissues where RIIβ dominates. They enable detection of protein expression, post-translational modifications, and interactions via techniques like Western blotting, immunohistochemistry, and co-immunoprecipitation. Research using PKAR2 antibodies has elucidated its involvement in adipocyte differentiation, neurotransmitter receptor trafficking, and tumorigenesis, as aberrant RIIβ expression is observed in certain cancers. Commercial antibodies are typically validated for specificity against conserved regions of RIIβ, distinguishing it from other regulatory subunits. Studies leveraging these reagents continue to advance understanding of cAMP-PKA pathways in disease mechanisms and therapeutic targeting.
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