首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔子基质金属蛋白酶9(MMP-9)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的MMP9含量。MMP9 即基质金属蛋白酶 9，在多种生理和病理过程中发挥作用。其背景在于它参与细胞外基质的降解与重塑。研究机制上，它可被多种因素激活，能调控肿瘤细胞的侵袭、转移，也和炎症等疾病相关，是疾病治疗和药物研发的潜在靶点。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，适用于体外实验中炎症机制研究、肿瘤细胞侵袭模型分析、药物干预效果评估等基础科研场景，为心血管疾病、纤维化病理、创伤愈合等研究领域提供高效检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：MMP9Matrix metalloproteinase-9 ELISA Kit; MMP-9 ELISA Kit; EC 3.4.24.35 ELISA Kit; 92 kDa gelatinase ELISA Kit; 92 kDa type IV collagenase ELISA Kit; Gelatinase B ELISA Kit; GELB ELISA Kit
缩写：MMP9
Uniprot No.：P41246
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MMP-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:20 | Average % | 99 | ||||
| Range % | 96-102 | |||||
| 1:40 | Average % | 85 | ||||
| Range % | 92-99 | |||||
| 1:80 | Average % | 101 | ||||
| Range % | 98-104 | |||||
| 1:160 | Average % | 85 | ||||
| Range % | 82-89 | |||||
回收率：
| The recovery of rabbit MMP-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 93-99 | ||||
| EDTA plasma (n=4) | 100 | 96-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 15000.1290.1270.128 7500.2260.2380.232 3750.3790.3700.375 187.50.5720.5650.569 940.7990.7850.792 470.9370.9870.962 23.51.1481.1681.158 01.6061.6341.620 | ||||||
货期：3-5 working days
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