首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔子基质金属蛋白酶3(MMP-3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MMP3含量。MMP3 即基质金属蛋白酶 3，它在细胞外基质的降解和重塑等生理过程中发挥重要作用。其异常表达与肿瘤侵袭转移、关节炎等疾病发展密切相关。研究机制主要聚焦于其对基质成分的作用、信号通路的影响等，为相关疾病治疗提供靶点思路。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于体外培养细胞、动物模型组织等科研场景中MMP-3的动态监测，为研究结缔组织代谢调控、炎症性疾病机制及药物干预效果提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：MMP3 ELISA Kit; Stromelysin-1 ELISA Kit; SL-1 ELISA Kit; EC 3.4.24.17 ELISA Kit; Matrix metalloproteinase-3 ELISA Kit; MMP-3 ELISA Kit; Transin-1 ELISA Kit
缩写：MMP3
Uniprot No.：P28863
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MMP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 84 | ||||
| Range % | 80-94 | |||||
| 1:10 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:20 | Average % | 93 | ||||
| Range % | 85-97 | |||||
| 1:40 | Average % | 94 | ||||
| Range % | 86-99 | |||||
回收率：
| The recovery of rabbit MMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 89-98 | ||||
| EDTA plasma (n=4) | 89 | 85-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 102.7942.6942.7442.593 52.2442.1442.1942.043 2.51.4241.3241.3741.223 1.250.8540.8240.8390.688 0.6250.5460.5360.5410.390 0.3120.3610.3510.3560.205 0.1560.2300.2200.2250.074 00.1520.1490.151 | ||||||
货期：3-5 working days
×
