首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠白三烯D4(LTD4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的LTD4含量。LTD4即白三烯D4，是一种重要的生物活性介质。它参与多种炎症和过敏反应，与哮喘、过敏性鼻炎等疾病密切相关。研究机制主要聚焦于其与受体结合后触发细胞内信号传导，导致气道收缩、炎症细胞浸润等一系列病理生理变化。试剂盒检测范围为7.8 pg/mL-500 pg/mL，本产品适用于基础科研中LTD4相关通路研究，如呼吸道疾病动物模型评估、炎症信号机制探索或抗炎药物筛选等实验场景，为体外实验提供稳定可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：LTD4
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Biochemicals
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat LTD4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 92 | |||||
| Range % | 88-97 | ||||||
| 1:2 | Average % | 103 | |||||
| Range % | 99-108 | ||||||
| 1:4 | Average % | 91 | |||||
| Range % | 85-97 | ||||||
| 1:8 | Average % | 94 | |||||
| Range % | 91-98 | ||||||
回收率：
| The recovery of rat LTD4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 96 | 92-101 | |||||
| EDTA plasma (n=4) | 92 | 87-98 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 5002.8432.9602.9022.799 2502.3882.3242.3562.253 1251.5731.5481.5611.458 62.50.8720.8840.8780.775 31.20.4450.4380.4420.339 15.60.3230.3100.3170.214 7.80.2150.2060.2110.108 00.1040.1020.103 | |||||||
货期：3-5 working days
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