首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠白三烯B4(LTB4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LT-B4含量。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于实验室环境中开展炎症相关疾病模型研究、药物干预效果评估或免疫调控机制探索等科研场景，尤其适合同步分析血液样本与组织局部炎症介质的表达水平，为深入解析LTB4在病理生理过程中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：LT-B4
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat LT-B4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||||||||
| Sample | Serum(n=4) | ||||||||||||
| 1:1 | Average % | 94 | |||||||||||
| Range % | 89-99 | ||||||||||||
| 1:2 | Average % | 97 | |||||||||||
| Range % | 91-103 | ||||||||||||
| 1:4 | Average % | 97 | |||||||||||
| Range % | 92-104 | ||||||||||||
| 1:8 | Average % | 93 | |||||||||||
| Range % | 88-97 | ||||||||||||
回收率：
| The recovery of rat LT-B4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||||||||
| Sample Type | Average % Recovery | Range | |||||||||||
| Serum (n=5) | 92 | 87-96 | |||||||||||
| EDTA plasma (n=4) | 97 | 93-106 | |||||||||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||
| pg/mlOD1OD2AverageCorrected10002.4422.5412.4922.3205001.8871.9861.9371.7652501.2821.2631.2731.1011250.8300.8210.8260.65462.50.5110.5020.5070.33531.20.3700.3610.3660.19415.60.2640.2590.2620.09000.1710.1720.172 | |||||||||||||
货期：3-5 working days
×
