首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠脂蛋白磷脂酶A2(Lp-PLA2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的Lp-PLA2含量。Lp-PLA2（脂蛋白相关磷脂酶A2）是一种与动脉粥样硬化和心血管疾病密切相关的标志物，由血管内膜中的巨噬细胞、T细胞和肥大细胞分泌。其水平高低与脑卒中和心血管疾病风险密切相关，通过水解氧化低密度脂蛋白中的氧化磷脂，生成脂类促炎物质，促进动脉粥样硬化。试剂盒检测范围为0.78 IU/mL-50 IU/mL，该产品适用于科研领域中对大鼠模型血清或血浆样本的Lp-PLA2水平监测，例如心血管疾病动物模型的病理机制研究、药物干预效果评估或炎症相关代谢调控实验，为体外实验提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：Lp-PLA2
Uniprot No.：Q5M7T7
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma
检测范围：0.78 IU/mL-50 IU/mL
灵敏度：0.195 IU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Lp-PLA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 92 | |||||
| Range % | 88-96 | ||||||
| 1:2 | Average % | 105 | |||||
| Range % | 100-110 | ||||||
| 1:4 | Average % | 86 | |||||
| Range % | 82-90 | ||||||
| 1:8 | Average % | 93 | |||||
| Range % | 89-98 | ||||||
回收率：
| The recovery of rat Lp-PLA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 90-100 | |||||
| EDTA plasma (n=4) | 88 | 84-92 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| IU/mlOD1OD2AverageCorrected 502.2032.3612.2822.152 251.9242.0401.9821.852 12.51.5121.4591.4861.356 6.250.9360.9020.9190.789 3.120.4770.4850.4810.351 1.560.3000.3180.3090.179 0.780.2410.2340.2380.108 00.1320.1270.130 | |||||||
货期：3-5 working days
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