首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠脂蛋白脂酶(LPL)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LPL含量。LPL（脂蛋白脂肪酶）是一种由多种组织产生的多功能酶，主要参与脂质和脂蛋白代谢。其研究机制涉及催化乳糜微粒和极低密度脂蛋白中的甘油三酯水解，产生脂肪酸和单酰甘油，供组织利用。LPL基因突变可能导致脂质代谢紊乱，与多种疾病如2型糖尿病、高血压、动脉硬化等关联。试剂盒检测范围为7.8 pg/mL-500 pg/mL，广泛用于基础科研领域，例如在动物模型中探究饮食干预、基因修饰或药物处理对脂代谢通路的影响，评估不同生理病理状态下LPL的活性变化及其与代谢综合征的关联机制，为心血管疾病、糖尿病等代谢相关疾病的机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Lpl ELISA Kit; Lipoprotein lipase ELISA Kit; LPL ELISA Kit; EC 3.1.1.34 ELISA Kit
缩写：LPL
Uniprot No.：Q06000
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat LPL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 93 | ||||
| Range % | 87-96 | |||||
| 1:400 | Average % | 102 | ||||
| Range % | 97-105 | |||||
| 1:800 | Average % | 97 | ||||
| Range % | 92-101 | |||||
| 1:1600 | Average % | 98 | ||||
| Range % | 93-101 | |||||
回收率：
| The recovery of rat LPL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 84-91 | ||||
| EDTA plasma (n=4) | 99 | 94-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected5002.5382.5122.5252.4132502.0141.9771.9961.8841251.4931.4641.4791.36762.50.8010.8170.8090.69731.20.4830.4660.4750.36315.60.2810.2720.2770.1657.80.1940.1880.1910.07900.1130.1110.112 | ||||||
货期：3-5 working days
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