首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：人兜甲蛋白(LOR)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的LOR含量。试剂盒检测范围为25 pg/mL-1600 pg/mL，适用于科研场景中皮肤生物学机制研究、疾病模型表皮分化评估或药物干预效果分析。科研人员可通过本产品定量检测不同实验条件下LOR的表达水平变化，为探索皮肤屏障功能调控、表皮异常分化机制及相关疾病发病机理提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：LOR ELISA Kit; LOR protein ELISA Kit; LORI_HUMAN ELISA Kit; Loricrin ELISA Kit; LRN ELISA Kit; MGC111513 ELISA Kit; OTTHUMP00000015823 ELISA Kit
缩写：LOR
Uniprot No.：P23490
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human LOR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 81-89 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 94-100 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 95-101 | |||||
| 1:8 | Average % | 87 | ||||
| Range % | 82-91 | |||||
回收率：
| The recovery of human LOR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 90-96 | ||||
| EDTA plasma (n=4) | 102 | 98-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 16002.4242.4682.4462.300 8001.5611.5981.5801.434 4000.9860.9460.9660.820 2000.6340.6570.6460.500 1000.4110.4350.4230.277 500.2950.2750.2850.139 250.2020.2130.2080.062 00.1450.1470.146 | ||||||
货期：3-5 working days
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