首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠层连蛋白/板层素(LN)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的LN含量。大鼠层连蛋白/板层素（LN）是细胞外基质的主要结构成分，对基膜的组装起关键作用。其在细胞表面形成网络结构，将细胞固定在基膜上，参与多种生理过程，如细胞粘附、增殖和分化。研究显示，LN在肝硬化等疾病中表达增加，可能与肝窦毛细血管化形成有关。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于基础医学研究中LN相关分子机制探索，如肝/肺纤维化模型评估、伤口愈合过程监测、肿瘤侵袭性研究等，为体外实验提供可靠的蛋白定量工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：LN
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat LN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 91 | ||||
| Range % | 87-99 | |||||
| 1:200 | Average % | 97 | ||||
| Range % | 93-105 | |||||
| 1:400 | Average % | 85 | ||||
| Range % | 82-89 | |||||
| 1:800 | Average % | 90 | ||||
| Range % | 85-98 | |||||
回收率：
| The recovery of rat LN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-95 | ||||
| EDTA plasma (n=4) | 95 | 89-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 40000.0690.0750.072 20000.1240.1250.125 10000.2050.2230.214 5000.3610.3400.351 2500.5930.6240.609 1250.8210.8580.840 62.51.1191.1161.118 02.1652.2162.191 | ||||||
货期：3-5 working days
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