Recombinant Human SDC4 protein

中文名： 龙豆糖核心蛋白(SDC4)重组蛋白
别名： SDC4；Syndecan-4

货号: PA1000-3096

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>98%SDS-PAGE.
种属	Human
靶点	SDC4
Uniprot No	P31431
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	19-145aa
氨基酸序列	ESIRETEVIDPQDLLEGRYFSGALPDDEDVVGPGQESDDFELSGSGDLDD LEDSMIGPEVVHPLVPLDNHIPERAGSGSQVPTEPKKLEENEVIPKRISP VEESEDVSNKVSMSSTVQGSNIFERTE
预测分子量	14 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是3篇关于SDC4重组蛋白的参考文献概览(注：文献为虚拟示例，实际研究需根据具体数据库检索)：

1. **"Recombinant Syndecan-4 Extracellular Domain Inhibits Tumor Angiogenesis in Vivo"**

*作者：Lee et al. (2020)*

摘要：研究团队通过大肠杆菌表达系统制备重组SDC4胞外域蛋白，发现其通过竞争性结合VEGF抑制肿瘤血管生成，为癌症治疗提供潜在策略。

2. **"Structural Analysis of Recombinant Human SDC4 Glycoprotein via Cryo-EM"**

*作者：Zhang et al. (2019)*

摘要：利用冷冻电镜解析重组人源SDC4蛋白的三维结构，揭示其硫酸肝素链与整合素相互作用的分子机制，为靶向药物设计提供结构基础。

3. **"SDC4 Recombinant Protein Enhances Wound Healing via ERK Signaling Pathway"**

*作者：Smith et al. (2021)*

摘要：通过哺乳动物细胞表达系统获得功能性重组SDC4.证实其通过激活ERK通路促进成纤维细胞迁移，加速皮肤创伤修复过程。

实际文献建议通过PubMed/Google Scholar检索关键词 "recombinant SDC4 protein" 或结合研究领域筛选(如癌症、纤维化等)。

背景信息

**Background of SDC4 Recombinant Protein**

SDC4 (Syndecan-4) is a transmembrane heparan sulfate proteoglycan belonging to the syndecan family, which plays critical roles in cell-matrix interactions, signaling, and tissue homeostasis. It consists of an extracellular domain modified with glycosaminoglycan (GAG) chains, a single transmembrane domain, and a short cytoplasmic tail that interacts with cytoskeletal and signaling proteins. SDC4 is ubiquitously expressed and is particularly important in regulating cell adhesion, migration, proliferation, and mechanotransduction, largely through its interactions with extracellular matrix (ECM) components like fibronectin and growth factors.

Recombinant SDC4 protein is engineered *in vitro* using expression systems such as mammalian cells, insect cells, or *E. coli*, depending on desired post-translational modifications (e.g., GAG chains). The purified protein retains functional domains, enabling studies on its biological activities without membrane-associated complexity. Researchers often utilize it to investigate SDC4-mediated pathways, including its role in activating protein kinase C alpha (PKCα) and regulating Rho GTPases, which influence cytoskeletal dynamics.

SDC4 is implicated in both physiological and pathological processes. It promotes wound healing by facilitating fibroblast-ECM interactions but also contributes to diseases like fibrosis, cancer metastasis, and inflammation. In cancer, SDC4 exhibits dual roles—either suppressing or promoting tumor progression depending on context—making it a potential therapeutic target. Recombinant SDC4 aids in drug discovery, antibody development, and mechanistic studies, particularly in dissecting its extracellular ligand-binding versus intracellular signaling functions.

Overall, SDC4 recombinant protein serves as a vital tool for unraveling the multifaceted roles of syndecans in health and disease, bridging structural biology with translational research.