首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠白介素33(IL-33)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL33含量。IL-33是一种前炎症细胞因子，属于IL-1家族，主要在细胞损伤时释放，诱导Th2型炎症反应。其与ST2受体结合后，激活下游信号通路，调节多种免疫细胞功能。IL-33在肿瘤、自身免疫疾病和过敏性疾病中发挥重要作用。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于基础科研中炎症机制研究、免疫调控模型分析以及药物干预效果评估等方向，例如在呼吸道炎症模型、自身免疫疾病或创伤修复实验中定量分析IL - 33的动态变化。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Il33Interleukin-33 ELISA kit; IL-33 ELISA kit
缩写：IL33
Uniprot No.：Q66H70
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-33 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 103 | ||||
| Range % | 98-108 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-101 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 83-95 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 93-104 | |||||
回收率：
| The recovery of rat IL-33 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 85-94 | ||||
| EDTA plasma (n=4) | 94 | 90-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 10002.9812.9982.9902.842 5001.9671.8731.9201.772 2501.3331.4271.3801.232 1250.8450.8350.8400.692 62.50.5650.5590.5620.414 31.20.3310.3480.3400.192 15.60.2250.2360.2310.083 00.1430.1530.148 | ||||||
货期：3-5 working days
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