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产品描述：人白介素33(IL-33)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液样本中的IL33含量。IL33，又称白细胞介素33，是一种前炎症细胞因子，属于IL-1超家族。它主要在细胞损伤或炎症时释放，通过与其受体ST2结合，激活细胞内信号通路，参与调节先天性和获得性免疫反应。IL33在多种疾病中发挥作用，如过敏性疾病、自身免疫疾病和癌症，其研究机制涉及IL33与多种免疫细胞相互作用，以及其在炎症和免疫调节中的双重作用。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，灵敏度为3.9 pg/mL。特别适用于探索IL - 33在免疫相关疾病动物模型、细胞因子网络调控机制或生物标志物筛选等基础研究中的应用，为炎症性疾病、自身免疫病等领域的科研工作提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：C9orf26 ELISA Kit; CHROMOSOME 9 OPEN READING FRAME 26 ELISA Kit; DKFZp586H0523 ELISA Kit; DVS27 ELISA Kit; DVS27 related protein ELISA Kit; IL 1F11 ELISA Kit; IL 33 ELISA Kit; IL-1F11 ELISA Kit; IL-33 ELISA Kit; IL1F11 ELISA Kit; IL33 ELISA Kit; IL33_HUMAN ELISA Kit; Interleukin 1 family member 11 ELISA Kit; Interleukin 33 ELISA Kit; INTERLEUKIN 33 NFHEV ELISA Kit; Interleukin 33 precursor ELISA Kit; Interleukin-1 family member 11 ELISA Kit; Interleukin-33 (109-270) ELISA Kit; Interleukin33 ELISA Kit; NF HEV ELISA Kit; NF-HEV ELISA Kit; NFEHEV ELISA Kit; NFHEV ELISA Kit; Nuclear factor for high endothelial venules ELISA Kit; Nuclear factor from high endothelial venules ELISA Kit; OTTHUMP00000021041 ELISA Kit; RP11 575C20.2 ELISA Kit
缩写：IL33
Uniprot No.：O95760
种属：Homo sapiens (Human)
样本类型：serum, plasma, urine
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL-33 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 80-94 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 90-99 | |||||
| 1:4 | Average % | 106 | ||||
| Range % | 92-115 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 86-100 | |||||
回收率：
| The recovery of human IL-33 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-99 | ||||
| EDTA plasma (n=4) | 97 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10001.8941.7521.8231.7285001.2751.1861.2311.1362500.7340.6980.7160.6211250.4210.4090.4150.32062.50.2450.2390.2420.14731.20.1650.1590.1620.06715.60.1120.1090.1110.01600.0950.0940.095 | ||||||
货期：3-5 working days
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