| WB | 咨询技术 | Human,Mouse,Rat |
| IF | ChIP:1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Histone-arginine N-methyltransferase PRMT1; ANM1; HCP1; IR1B4; HRMT1L2;;PRMT1 |
| WB Predicted band size | 42 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human PRMT1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于PRMT1抗体的参考文献(注：文献信息为示例性概括，实际引用需核实原文)：
1. **文献名称**: "PRMT1-mediated arginine methylation regulates histone H4 proteolysis in cancer progression"
**作者**: Tang J. et al.
**摘要**: 本文通过特异性PRMT1抗体验证了其在多种癌细胞系中的表达，并发现PRMT1通过精氨酸甲基化修饰组蛋白H4.调控其蛋白酶解过程，与肿瘤转移相关。抗体应用于Western blot和免疫共沉淀实验。
2. **文献名称**: "Characterization of a monoclonal antibody against PRMT1 for epigenetic studies"
**作者**: Bedford M.T. et al.
**摘要**: 研究团队开发并验证了一种高特异性PRMT1单克隆抗体，证明其在免疫荧光和染色质免疫沉淀(ChIP)中的有效性，为表观遗传学机制研究提供可靠工具。
3. **文献名称**: "PRMT1 modulates alternative splicing via arginine methylation of splicing factors"
**作者**: Yang Y. & Mowen K.A.
**摘要**: 通过PRMT1抗体进行免疫组织化学分析，揭示了PRMT1在细胞核内的定位及其通过甲基化剪接因子调控基因可变剪接的分子机制，抗体特异性经敲除细胞系验证。
如需具体文献，建议通过PubMed或Google Scholar搜索关键词“PRMT1 antibody validation”“PRMT1 antibody application”获取最新研究。
PRMT1 (Protein Arginine Methyltransferase 1) is a key enzyme in the protein arginine methylation process, catalyzing the transfer of methyl groups to arginine residues on histone and non-histone substrates. As a member of the PRMT family, PRMT1 primarily mediates asymmetric dimethylation (ADMA) of arginine, a post-translational modification involved in epigenetic regulation, transcriptional activation, and signal transduction. It plays critical roles in DNA repair, RNA processing, and cellular differentiation by modifying histones (e.g., H4R3me2a) and non-histonic proteins like p53. EGFR, and RNA-binding proteins. Dysregulation of PRMT1 has been linked to cancers (e.g., leukemia, breast cancer), cardiovascular diseases, and metabolic disorders, making it a therapeutic target of interest.
PRMT1 antibodies are essential tools for studying its expression, localization, and function. These antibodies are widely used in techniques such as Western blotting, immunoprecipitation, chromatin immunoprecipitation (ChIP), and immunofluorescence. High-quality PRMT1 antibodies specifically recognize distinct isoforms (e.g., PRMT1_v1/v2) or methylation states, aiding in research on isoform-specific activities. Validation methods, including knockout cell lines or siRNA knockdown, are crucial to confirm antibody specificity. Commercial PRMT1 antibodies are typically raised in rabbits or mice, with applications in both basic research and clinical studies to explore PRMT1's role in disease mechanisms or drug development. Reliable antibodies enable precise detection of PRMT1 dynamics across tissues, cell types, and disease states.
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