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产品描述：大鼠白介素1受体拮抗剂(IL1Ra)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL1RN含量。IL1RN是重要靶点。它属于白细胞介素-1受体拮抗剂，可竞争结合IL-1受体。背景上，其失衡与多种炎症性疾病相关。研究机制方面，主要是通过阻断IL-1信号通路，抑制炎症反应，在炎症疾病治疗研究里具有重要价值。试剂盒检测范围为125 pg/mL-8000 pg/mL，适用于科研领域中对炎症机制、自身免疫疾病模型或药物干预效果的动态研究。为探索IL - 1信号通路调控、评估实验动物体内抗炎反应强度提供了可靠工具，广泛应用于基础医学、药理学及生物标志物研究等领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Il1rn ELISA Kit; Il-1raInterleukin-1 receptor antagonist protein ELISA Kit; IL-1RN ELISA Kit; IL-1ra ELISA Kit; IRAP ELISA Kit; IL1 inhibitor ELISA Kit
缩写：IL1RN
Uniprot No.：P25086
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：125 pg/mL-8000 pg/mL
灵敏度：31.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-1ra in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 102 | ||||
| Range % | 98-105 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-102 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 90-101 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 88-99 | |||||
回收率：
| The recovery of rat IL-1ra spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 92-104 | ||||
| EDTA plasma (n=4) | 89 | 82-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected80002.2592.2692.2642.14840001.5541.5971.5761.46020001.0401.0681.0540.93810000.6250.6720.6490.5335000.3980.3710.3850.2692500.2790.2740.2770.1611250.1810.1890.1850.06900.1140.1180.116 | ||||||
货期：3-5 working days
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