| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | APPS; Cathepsin B heavy chain; Cathepsin B1; CathepsinB; CPSB; CTSB; cysteine protease; Preprocathepsin B; ;Cathepsin B |
| WB Predicted band size | 38 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human Cathepsin B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Cathepsin B抗体的3篇参考文献示例(内容基于公开研究整理，具体引用时请核实原文)：
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1. **文献名称**：*Cathepsin B: Multiple roles in cancer*
**作者**：Gocheva, V., & Joyce, J.A.
**摘要**：该综述总结了Cathepsin B在肿瘤微环境中的功能，包括其在细胞外基质降解、肿瘤侵袭和转移中的作用。文中提到利用特异性抗体检测Cathepsin B在癌细胞及肿瘤相关巨噬细胞中的表达，为靶向治疗提供依据。
2. **文献名称**：*Role of cathepsin B in Alzheimer's disease: Immunohistochemical study in human brain tissue*
**作者**：Hook, V.Y., et al.
**摘要**：研究通过免疫组化技术(使用Cathepsin B特异性抗体)分析了阿尔茨海默病患者脑组织中该蛋白酶的分布，发现其与β-淀粉样斑块沉积相关，提示其可能参与神经退行性病变过程。
3. **文献名称**：*Development of a monoclonal antibody against human cathepsin B for cancer diagnosis*
**作者**：Berdowska, I., et al.
**摘要**：文章描述了一种新型抗人Cathepsin B单克隆抗体的开发与验证，该抗体在结直肠癌组织样本中表现出高灵敏度和特异性，可用于临床病理诊断及预后评估。
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如需具体文献，建议通过PubMed或Google Scholar以关键词“Cathepsin B antibody”检索最新或高引论文。
Cathepsin B is a lysosomal cysteine protease belonging to the papain family, primarily involved in intracellular protein degradation and processing. It plays critical roles in normal cellular processes such as apoptosis, antigen presentation, and tissue remodeling. However, dysregulated Cathepsin B expression or activity has been implicated in various pathological conditions, including cancer metastasis, neurodegenerative diseases (e.g., Alzheimer’s), inflammatory disorders, and infectious diseases. Its overexpression in tumors correlates with enhanced invasion, angiogenesis, and chemotherapy resistance, making it a potential therapeutic target and biomarker.
Antibodies targeting Cathepsin B are essential tools for studying its expression, localization, and function in both physiological and disease contexts. These antibodies enable detection through techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. Specificity validation is crucial, as Cathepsin B shares structural homology with other cathepsin family members. High-quality antibodies help distinguish between the inactive pro-enzyme (∼40 kDa) and active mature forms (∼25-30 kDa), providing insights into its activation status. Researchers also use these antibodies to explore therapeutic strategies, such as inhibiting Cathepsin B in cancer or modulating its activity in lysosomal storage disorders. Cross-reactivity with orthologs in common model organisms (e.g., mouse, rat) further supports translational studies. Overall, Cathepsin B antibodies are vital for advancing research into its dual roles in health and disease.
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