Mouse Interleukin 1β,IL-1β ELISA Kit

货号: IPD-E08054m

Price： ￥2500

48T 96T

数量：

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产品描述：
小鼠白介素1β (IL-1β)酶联免疫试剂盒，双抗夹心法ELISA试剂盒，定量检测样本中的IL1B含量。IL1B，即白细胞介素1β，是一种促炎细胞因子，主要由巨噬细胞和树突状细胞产生。它在炎症和免疫反应中起关键作用，通过激活信号传导途径，如MyD88和Toll样受体，调节多种细胞活动，包括细胞增殖、分化和凋亡。IL1B的研究涉及其在先天免疫中的重要作用及其与多种疾病的关系。适用于炎症性疾病模型构建、免疫调控机制研究、药物抗炎效果评估等场景，例如通过检测实验动物炎症模型中IL - 1β的动态变化，或分析体外培养细胞受刺激后的细胞因子释放水平。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Interleukin-1 beta ELISA Kit; IL-1 beta ELISA Kit
缩写：
IL1B
Uniprot No.：
P10749
种属：
Mus musculus (Mouse)
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	88
	Range %	82-95
1:2	Average %	93
	Range %	88-98
1:4	Average %	96
	Range %	94-102
1:8	Average %	94
	Range %	90-100

回收率：

The recovery of mouse IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	87-99
EDTA plasma (n=4)	93	88-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.758	2.641	2.700	2.586
1000	1.438	1.587	1.513	1.399
500	0.833	0.869	0.851	0.737
250	0.474	0.477	0.476	0.362
125	0.350	0.375	0.363	0.249
62.5	0.267	0.253	0.260	0.146
31.25	0.165	0.186	0.176	0.062
0	0.119	0.108	0.114

A micro ELISA plate --- The 96-well plate has been pre-coated with anti-mouse IL-1β This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
Biotin-labeled IL-1β antibody (100 x concentrate) 1 x 120 μl ---Act as the detection antibody.
HRP-avidin (100 x concentrate) 1 x 120 μl --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
Biotin-antibody Diluent 1 x 15 ml ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
HRP-avidin Diluent 1 x 15 ml ---Dilute the high concentration HRP-avidin solution to an appropriate solution.

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate

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