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Human Interleukin 1β,IL-1β ELISA Kit

  • 中文名： 人白介素1β (IL-1β)ELISA Kit
货号: IPD-E08053h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人白介素1β (IL-1β)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL1B含量。IL1B即白细胞介素-1β，是一种重要的炎性细胞因子。背景上，它在多种炎症反应和免疫调节中起关键作用。研究机制方面，其参与激活免疫细胞、调节炎症信号通路等，与感染、自身免疫病等多种疾病发生发展密切相关。试剂盒检测范围为7.8 pg/ml- 500 pg/ml，灵敏度为1.95 pg/ml。支持科研人员在炎症机制探索、疾病模型建立、药物抗炎效果评估等方向开展定量分析，尤其适合需要检测生物体液或组织样本中低丰度IL-1β表达的应用场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Catabolin ELISA Kit; H1 ELISA Kit; IFN beta inducing factor ELISA Kit; IL 1 ELISA Kit; IL 1 beta ELISA Kit; IL-1 beta ELISA Kit; IL1 ELISA Kit; IL1 BETA ELISA Kit; IL1B ELISA Kit; IL1B_HUMAN ELISA Kit; IL1F2 ELISA Kit; Interleukin 1 beta ELISA Kit; Interleukin 1 beta precursor ELISA Kit; interleukin 1; beta ELISA Kit; Interleukin-1 beta ELISA Kit; OAF ELISA Kit; Osteoclast activating factor ELISA Kit; OTTHUMP00000162031 ELISA Kit; Preinterleukin 1 beta ELISA Kit; Preinterleukin beta ELISA Kit; Pro interleukin 1 beta ELISA Kit
  • 缩写：
    IL1B
  • Uniprot No.：
    P01584
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    7.8 pg/ml - 500 pg/ml
  • 灵敏度：
    1.95 pg/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    90


    Range %

    80-95


    1:2

    Average %

    96


    Range %

    91-105


    1:4

    Average %

    107


    Range %

    103-112


    1:8

    Average %

    93


    Range %

    86-98


  • 回收率：

    The recovery of human IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    95

    89-98


    EDTA plasma (n=4)

    92

    88-96


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    500

    2.439

    2.604

    2.522

    2.343


    250

    1.493

    1.467

    1.480

    1.301


    125

    0.864

    0.858

    0.861

    0.683


    62.5

    0.566

    0.589

    0.578

    0.399


    31.2

    0.378

    0.361

    0.370

    0.191


    15.6

    0.309

    0.293

    0.301

    0.122


    7.8

    0.267

    0.254

    0.261

    0.082


    0

    0.182

    0.175

    0.179




  • 货期：
    3-5 working days

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