首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠白介素18(IL-18)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的IL18含量。IL18是一种促炎细胞因子，在免疫调节中起关键作用。其研究机制涉及与IL18R1和IL18RAP结合，形成信号复合物，激活NF-κB，导致炎症介质合成。IL18在肿瘤免疫治疗中具有潜在应用价值。试剂盒检测范围为1.56 pg/mL-100 pg/mL，适用于科研领域中对炎症反应机制、免疫调节研究或药物干预效果的评估，例如在实验性自身免疫性脑脊髓炎（EAE）模型、败血症模型或肥胖相关研究中定量分析IL - 18的动态变化。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Il18 ELISA Kit; Igif ELISA Kit; Interleukin-18 ELISA Kit; IL-18 ELISA Kit; Interferon gamma-inducing factor ELISA Kit; IFN-gamma-inducing factor ELISA Kit; Interleukin-1 gamma ELISA Kit; IL-1 gamma ELISA Kit
缩写：IL18
Uniprot No.：P70380
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：1.56 pg/mL-100 pg/mL
灵敏度：0.39 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 82-94 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 91-103 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 88-99 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of mouse IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-100 | ||||
| EDTA plasma (n=4) | 99 | 94-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 1002.6462.5522.5992.484 502.2182.1862.2022.087 251.8741.8041.8391.724 12.51.3321.3291.3311.216 6.250.9250.9010.9130.798 3.120.5270.4990.5130.398 1.560.2610.2780.2700.155 00.1190.1110.115 | ||||||
货期：3-5 working days
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