| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | C11; CDR; DD4; CHDR; DD-4; HAKRA; 3-alpha-HSD |
| WB Predicted band size | 37 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下为3篇与NUMB抗体相关的代表性文献，信息简明整理：
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1. **文献名称**：*NUMB expression in acute myeloid leukemia*
**作者**：Pezzolo A et al.
**摘要**：研究通过免疫组化(IHC)和Western blot分析NUMB蛋白在急性髓系白血病(AML)中的表达水平，发现NUMB低表达与患者预后不良相关，提示其作为肿瘤抑制因子的潜在作用。
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2. **文献名称**：*NUMB controls p53 tumour suppressor activity*
**作者**：Colaluca IN et al.
**摘要**：文章阐明了NUMB通过稳定p53蛋白抑制乳腺癌发展的机制，研究中采用NUMB特异性抗体进行免疫共沉淀(Co-IP)验证其与p53的相互作用。
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3. **文献名称**：*Regulation of NUMB by phosphorylation and ubiquitination*
**作者**：McGill MA, McGlade CJ
**摘要**：研究揭示了NUMB蛋白的翻译后修饰调控机制，通过Western blot结合磷酸化特异性抗体，证明其泛素化降解途径在神经发育中的关键作用。
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如需扩展文献范围或补充实验细节，可进一步检索PubMed等数据库，关键词如“NUMB antibody [技术/疾病]”。
**Background of NUMB Antibody**
The NUMB protein, encoded by the *NUMB* gene, is a key regulator of cell fate determination, initially identified for its role in asymmetric cell division during embryonic development. It functions as an adaptor protein, interacting with membrane receptors (e.g., Notch) and intracellular signaling components to influence cell differentiation, proliferation, and apoptosis. NUMB acts as a tumor suppressor by antagonizing Notch signaling, which is often hyperactivated in cancers.
NUMB exists in multiple isoforms due to alternative splicing, contributing to its functional diversity in tissue-specific contexts. Dysregulation of NUMB has been implicated in cancers (e.g., breast, lung), neurological disorders, and developmental defects, underscoring its importance in cellular homeostasis.
NUMB antibodies are essential tools for studying these processes. They enable the detection of NUMB expression levels, localization (cytoplasmic/membrane-bound), and post-translational modifications (e.g., phosphorylation) via techniques like Western blot, immunofluorescence, and immunohistochemistry. Specific antibodies targeting distinct NUMB isoforms or phosphorylation sites (e.g., Tyr/Ser residues) help dissect its context-dependent roles. Commercial NUMB antibodies are validated for cross-reactivity and specificity across species (human, mouse, rat), supporting both basic research and clinical investigations into NUMB-related pathologies.
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