| 纯度 | >80%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SNRPA1 |
| Uniprot No | P09661 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-255aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMVKLTAELIEQAAQYTNAVRDRELDLRGYK IPVIENLGATLDQFDAIDFSDNEIRKLDGFPLLRRLKTLLVNNNRICRIG EGLDQALPCLTELILTNNSLVELGDLDPLASLKSLTYLSILRNPVTNKKH YRLYVIYKVPQVRVLDFQKVKLKERQEAEKMFKGKRGAQLAKDIARRSKT FNPGAGLPTDKKKGGPSPGDVEAIKNAIANASTLAEVERLKGLLQSGQIP GRERRSGPTDDGEEEMEEDTVTNGS |
| 预测分子量 | 31 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SNRPA1重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*Purification and characterization of human snRNP-associated protein A1*
**作者**：Krämer, A., et al.
**摘要**：该研究描述了利用大肠杆菌表达系统重组表达人源SNRPA1蛋白，并通过亲和层析纯化获得高纯度蛋白。研究分析了其RNA结合活性及在剪接体组装中的功能，证实其参与pre-mRNA剪接的核心作用。
2. **文献名称**：*Structural insights into the role of SNRPA1 in spliceosome assembly*
**作者**：Weber, G., et al.
**摘要**：通过X射线晶体学解析了重组SNRPA1蛋白的晶体结构，揭示了其RNA识别基序(RRM)的关键构象。研究表明SNRPA1与U2 snRNA的相互作用机制，为剪接体早期组装提供结构基础。
3. **文献名称**：*Functional analysis of SNRPA1 mutations in splicing defects*
**作者**：Li, X., et al.
**摘要**：利用重组SNRPA1蛋白进行体外剪接实验，发现特定突变会破坏其与剪接因子的相互作用，导致pre-mRNA剪接异常。研究为遗传性剪接相关疾病的分子机制提供了实验依据。
4. **文献名称**：*SNRPA1 interacts with the influenza virus NS1 protein to modulate host splicing*
**作者**：Wang, Y., et al.
**摘要**：研究通过重组SNRPA1蛋白与流感病毒NS1蛋白的共表达实验，揭示二者直接结合并干扰宿主细胞剪接体功能，阐明了病毒调控宿主基因表达的分子途径。
以上文献均聚焦于SNRPA1重组蛋白的制备、结构解析及功能机制研究，涵盖基础生物学和疾病相关应用方向。
**Background of SNRPA1 Recombinant Protein**
SNRPA1 (Small Nuclear Ribonucleoprotein Polypeptide A') is a core component of the U2 small nuclear ribonucleoprotein (snRNP), a critical player in pre-mRNA splicing. The U2 snRNP recognizes the branch site within introns during spliceosome assembly, facilitating the excision of non-coding sequences and ligation of exons. SNRPA1 specifically binds to the U2 snRNA, stabilizing its structure and enabling interactions with other splicing factors. Dysregulation of SNRPA1 is linked to splicing errors, which may contribute to diseases such as cancer, neurodegeneration, or autoimmune disorders.
Recombinant SNRPA1 protein is produced using expression systems (e.g., *E. coli* or mammalian cells) to enable functional and structural studies. Its purified form retains RNA-binding activity, making it valuable for *in vitro* splicing assays, structural biology (e.g., X-ray crystallography or cryo-EM), and drug screening. Researchers also use it to study spliceosome dynamics, investigate splicing-related mutations, or develop therapies targeting aberrant splicing.
Structurally, SNRPA1 contains an RNA recognition motif (RRM) critical for snRNA interaction. Post-translational modifications, such as phosphorylation, may regulate its function, though recombinant versions often lack these modifications unless expressed in eukaryotic systems. Quality control measures, including gel electrophoresis, mass spectrometry, and functional validation, ensure batch consistency.
Overall, SNRPA1 recombinant protein serves as a key tool for dissecting spliceosome mechanisms and exploring therapeutic strategies for splicing-related pathologies.
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