首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠白介素13(IL-13)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的IL13含量。IL-13是一种由T细胞、肥大细胞等分泌的细胞因子。它在过敏反应、组织纤维化等病理过程中发挥重要作用。研究机制方面，IL-13通过与其受体结合，激活下游信号通路，调控基因表达，进而影响细胞增殖、分化及炎症因子释放等。试剂盒检测范围为7.8 pg/mL-500 pg/mL，该产品适用于探索Th2型免疫调控机制、评估药物干预对炎症通路的影响，或通过检测实验动物模型（如哮喘或寄生虫感染模型）中IL - 13的动态变化，揭示其与病理进程的关联性。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Il13 ELISA Kit; Il-13 ELISA Kit; Interleukin-13 ELISA Kit; IL-13 ELISA Kit; T-cell activation protein P600 ELISA Kit
缩写：IL13
Uniprot No.：P42203
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 88-93 | |||||
| 1:2 | Average % | 104 | ||||
| Range % | 99-107 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 88-97 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of rat IL-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 94 | 91-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected5001.8541.7661.8101.7272501.1171.1061.1121.0291250.7280.7430.7360.65362.50.5170.5340.5260.44331.20.3030.3120.3080.22515.60.2100.2020.2060.1237.80.1710.1640.1680.08500.0840.0820.083 | ||||||
本试剂盒所含材料：
A micro ELISA plate --The 96-well plate has been pre-coated with an anti-rat IL-13 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled IL-13 antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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