| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CAK; CCNH; CDK-activating kinase; cyclin H; cyclin-dependent kinase-activating kinase; Cyclin-H; MO15-associated protein; p34; p37;;Cyclin H |
| WB Predicted band size | 38 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human Cyclin H |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于CyclinH抗体的3篇参考文献示例(文献为虚构，供参考)：
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1. **文献名称**: "CyclinH modulates CDK7 kinase activity in the CAK complex"
**作者**: Smith A, et al.
**摘要**: 该研究利用CyclinH特异性抗体进行免疫共沉淀和Western blot分析，发现CyclinH与CDK7在CAK(CDK激活激酶)复合体中的结合对RNA聚合酶II的磷酸化至关重要，揭示了其在细胞周期调控中的关键作用。
2. **文献名称**: "Expression profiling of CyclinH in human cancers using a novel monoclonal antibody"
**作者**: Lee B, et al.
**摘要**: 作者开发了一种高特异性抗CyclinH单克隆抗体，并通过免疫组化分析发现CyclinH在多种肿瘤组织中异常高表达，提示其可能作为癌症诊断的生物标志物。
3. **文献名称**: "CyclinH-CDK7 interaction with TFIIH: Insights from antibody-mediated inhibition assays"
**作者**: Garcia C, et al.
**摘要**: 研究通过CyclinH抗体阻断实验，证明CyclinH-CDK7复合体与转录因子TFIIH的相互作用对DNA损伤修复和转录起始具有双重调控功能，为靶向治疗提供了理论依据。
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注：以上文献为示例性质，实际引用时请以真实发表的论文为准。建议通过PubMed或Google Scholar搜索关键词“CyclinH antibody”获取最新研究。
Cyclin H is a regulatory subunit of the cyclin-dependent kinase (CDK)-activating kinase (CAK) complex, which primarily associates with CDK7 and MAT1. This ternary complex plays dual roles in cell cycle regulation and transcription. In the cell cycle, Cyclin H/CDK7 phosphorylates and activates CDKs (e.g., CDK1. CDK2. CDK4. CDK6) that drive progression through the G1/S and G2/M phases. In transcription, the complex functions as a component of the general transcription factor TFIIH, facilitating RNA polymerase II-mediated gene expression by phosphorylating its C-terminal domain. Dysregulation of Cyclin H expression or activity has been linked to cancer, neurodegenerative disorders, and developmental defects, making it a subject of interest in both basic and clinical research.
Antibodies targeting Cyclin H are essential tools for studying its expression, localization, and interactions. They are widely used in techniques like Western blotting, immunofluorescence, immunoprecipitation, and immunohistochemistry to investigate Cyclin H's roles in cell cycle checkpoints, transcriptional regulation, and DNA repair mechanisms. Such antibodies also aid in exploring Cyclin H's potential as a therapeutic target, particularly in cancers where CDK7 inhibition is being evaluated. Validation of Cyclin H antibodies typically involves testing specificity using knockout cell lines or siRNA-mediated knockdown. Given its conserved function across eukaryotes, these antibodies have applications in diverse model organisms, further supporting their utility in mechanistic and translational studies.
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