首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：兔子免疫球蛋白E(IgE)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的IgE含量。IgE即免疫球蛋白E，是介导Ⅰ型超敏反应的抗体，在过敏反应、寄生虫感染等过程中发挥重要作用。研究机制上，其可与肥大细胞和嗜碱性粒细胞表面受体结合，当变应原再次入侵，结合IgE后促使细胞释放炎症介质，引发过敏症状。试剂盒检测范围为7.8 pg/mL-2000 pg/mL，适用于科研场景中兔模型过敏性炎症机制探索、寄生虫感染免疫动态监测、疫苗或药物干预实验的疗效评估等研究，尤其适合需要高灵敏度检测微量样本的应用需求。试剂盒兼容血清与血浆样本，为体外实验提供便捷的检测方案，满足基础研究中对免疫指标定量分析的要求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IgE
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma
检测范围：7.8 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IgE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 91 | ||||
| Range % | 84-96 | |||||
| 1:2000 | Average % | 89 | ||||
| Range % | 82-96 | |||||
| 1:4000 | Average % | 93 | ||||
| Range % | 87-100 | |||||
| 1:8000 | Average % | 94 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of rabbit IgE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-96 | ||||
| EDTA plasma (n=4) | 88 | 85-91 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 20000.2180.2200.219 5000.3530.3540.354 1250.6220.6340.628 31.251.5231.4361.480 7.82.3892.2262.308 03.1273.2443.186 | ||||||
货期：3-5 working days
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