| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ESRRA; ERRa; ERR1; HERR1; NR3B1; Steroid hormone receptor ERR1; ERR-alpha; ERRalpha; ESRL1; Estrogen receptor-like 1; Estrogen-related receptor alpha;;ERR alpha |
| WB Predicted band size | Calculated MW: 46 kDa ; Observed MW: 50 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human ERR alpha |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Estrogen-Related Receptor Alpha(ERRα)抗体的3篇参考文献，包含文献名称、作者及简要摘要：
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1. **"Validation of Antibodies for the Specific Detection of Human, Mouse, and Rat Estrogen-Related Receptor Alpha (ERRα)"**
*Schreiber SN, et al. (2004)*
摘要：该研究验证了多种ERRα抗体的特异性，通过siRNA敲低及基因敲除模型证实抗体在Western blot、免疫组化及ChIP实验中的可靠性，并探讨了ERRα在能量代谢中的调控作用。
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2. **"ERRα regulates metabolic genes and modulates mitochondrial biogenesis in cancer cells"**
*Devarakonda S, et al. (2011)*
摘要：研究利用ERRα特异性抗体进行染色质免疫沉淀(ChIP-seq)，揭示了ERRα直接结合代谢相关基因启动子，调控线粒体功能及肿瘤细胞代谢重编程的分子机制。
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3. **"Estrogen-Related Receptor Alpha (ERRα) as a Therapeutic Target in Triple-Negative Breast Cancer"**
*Bianco S, et al. (2019)*
摘要：通过免疫组化及Western blot分析，发现ERRα在三阴性乳腺癌中高表达且与不良预后相关;研究利用特异性抗体证明靶向ERRα可抑制肿瘤生长，提示其作为治疗靶点的潜力。
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如需更多文献或具体应用场景(如抗体品牌、实验方法)，可进一步补充信息。
Estrogen-related receptor alpha (ERRα), encoded by the ESRRA gene, is a member of the nuclear receptor superfamily of transcription factors. Although structurally related to estrogen receptors (ERs), ERRα does not bind natural estrogens. It regulates genes involved in diverse metabolic pathways, mitochondrial biogenesis, energy homeostasis, and cellular differentiation. ERRα is highly expressed in tissues with elevated energy demands, such as the heart, skeletal muscle, and brain, and plays critical roles in adaptive responses to metabolic stress. Dysregulation of ERRα has been implicated in cancer progression, metabolic disorders (e.g., obesity, diabetes), and neurodegenerative diseases.
ERRα antibodies are essential tools for studying its expression, localization, and molecular interactions. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). Specific antibodies target distinct epitopes (e.g., N-terminal, C-terminal domains) to assess protein levels, post-translational modifications, or DNA-binding activity. Validation via knockout controls or siRNA is crucial due to potential cross-reactivity with homologous receptors (ERRβ/γ).
Research utilizing ERRα antibodies has advanced understanding of its role in tumor metabolism, where it drives aerobic glycolysis and lipogenesis in cancers. These antibodies also aid in exploring ERRα as a therapeutic target, with inhibitors under investigation for cancer and metabolic diseases. Reliable antibodies remain pivotal for unraveling ERRα's complex regulatory networks and translational potential.
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