| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ACACA kinase; Acetyl-CoA carboxylase kinase; AMPK alpha 2 chain; AMPK subunit alpha-2; AMPK2; AMPKalpha2; HMGCR kinase; PRKAA; PRKAA2;;p-AMPK alpha 1/2 (T183/T172) |
| WB Predicted band size | Calculated MW: 64,62 kDa ; Observed MW: 62 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human AMPK alpha 1 around the phosphorylation site of T183 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于Phospho-AMPKα(Thr172)抗体的经典参考文献，按研究内容分类简要概括：
1. **文献名称**：*The AMP-activated protein kinase cascade—a unifying system for energy control*
**作者**：Hardie, D.G., et al.
**摘要**：系统综述AMPK的激活机制，指出Thr172磷酸化是AMPK活化的关键步骤，并强调使用Phospho-AMPKα(Thr172)抗体检测AMPK活性状态的方法学应用。
2. **文献名称**：*LKB1 is the upstream kinase in the AMP-activated protein kinase cascade*
**作者**：Shaw, R.J., et al.
**摘要**：通过基因敲除和免疫印迹实验(使用Phospho-AMPKα(Thr172)抗体)，证实LKB1是AMPK的Thr172磷酸化激酶，揭示了其在能量应激响应中的核心作用。
3. **文献名称**：*Calmodulin-dependent protein kinase kinase-β acts upstream of AMP-activated protein kinase in mammalian cells*
**作者**：Hawley, S.A., et al.
**摘要**：利用Phospho-AMPKα(Thr172)抗体验证CaMKKβ对AMPK的磷酸化激活，证明其在钙信号通路中调控AMPK活性的新机制。
4. **文献名称**：*The kinase LKB1 mediates glucose homeostasis in liver and therapeutic effects of metformin*
**作者**：Woods, A., et al.
**摘要**：通过免疫沉淀和Western blot(使用Thr172磷酸化特异性抗体)，阐明二甲双胍通过LKB1-AMPK通路改善糖代谢的分子机制，强调该抗体在代谢疾病研究中的关键作用。
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这些文献均通过Phospho-AMPKα(Thr172)抗体验证了AMPK活性调控的分子机制，覆盖能量代谢、药物作用和疾病模型等领域。
The Phospho-AMPKα (Thr172) antibody is a critical tool for studying the activation status of AMP-activated protein kinase (AMPK), a central regulator of cellular energy homeostasis. AMPK, a heterotrimeric enzyme composed of α, β, and γ subunits, is activated under low-energy conditions (e.g., ATP depletion) via phosphorylation at Thr172 within the α subunit’s catalytic domain. This phosphorylation, mediated by upstream kinases like LKB1 or CaMKKβ, is essential for AMPK’s ability to restore energy balance by inhibiting anabolic pathways (e.g., lipid/cholesterol synthesis) and promoting catabolic processes (e.g., glycolysis, fatty acid oxidation).
The Phospho-AMPKα (Thr172) antibody specifically detects AMPKα phosphorylated at Thr172. serving as a marker for AMPK activation. It is widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate AMPK signaling in metabolic disorders (e.g., diabetes, obesity), cancer, aging, and exercise physiology. Researchers employ this antibody to assess AMPK activity in response to stimuli such as nutrient deprivation, pharmacological agents (e.g., metformin, AICAR), or genetic manipulations.
Validation often includes testing in AMPK-deficient models or after treatment with AMPK activators/inhibitors to confirm specificity. Cross-reactivity with related kinases (e.g., phosphorylated Akt) should be ruled out. Its applications extend to preclinical studies evaluating therapeutic strategies targeting AMPK pathways. This antibody has become indispensable for elucidating AMPK’s role in health and disease, bridging molecular mechanisms to physiological outcomes.
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