Recombinant Human SERPINC1 protein

中文名： 丝氨蛋白酶抑制因子C1(SERPINC1)重组蛋白
别名： SERPINC1;AT3;Antithrombin-III

货号: PA1000-2889

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>85%SDS-PAGE.
种属	Human
靶点	SERPINC1
Uniprot No	P01008
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-464aa
氨基酸序列	MYSNVIGTVTSGKRKVYLLSLLLIGFWDCVTCHGSPVDICTAKPRDIPMNPMCIYRSPEKKATEDEGSEQKIPEATNRRVWELSKANSRFATTFYQHLADSKNDNDNIFLSPLSISTAFAMTKLGACNDTLQQLMEVFKFDTISEKTSDQIHFFFAKLNCRLYRKANKSSKLVSANRLFGDKSLTFNETYQDISELVYGAKLQPLDFKENAEQSRAAINKWVSNKTEGRITDVIPSEAINELTVLVLVNTIYFKGLWKSKFSPENTRKELFYKADGESCSASMMYQEGKFRYRHVAEGTQVLELPFKGDDITMVLILPKPEKSLAKVEKELTPEVLQEWLDELEEMMLVVHMPRFRIEDGFSLKEQLQDMGLVDLFSPEKSKLPGIVAEGRDDLYVSDAFHKAFLEVNEEGSEAAASTAVVIAGRSLNPNRVTFKANRPFLVFIREVPLNTIIFMGRVANPCVK
预测分子量	81.5 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于SERPINC1重组蛋白的3篇参考文献示例(注：文献信息为示例性概括，实际引用请通过学术数据库核实)：

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1. **文献名称**: *"Production and Characterization of Recombinant Human Antithrombin III in Mammalian Cells"*

**作者**: Zhang et al.

**摘要**: 研究报道了在CHO细胞中高效表达重组人抗凝血酶III(ATIII)的方法，通过优化培养条件提高产量，并证实重组蛋白的糖基化修饰和抗凝血活性与天然血浆来源的ATIII相似。

2. **文献名称**: *"Functional Analysis of Recombinant SERPINC1 Mutants in Thrombosis Models"*

**作者**: Johnson et al.

**摘要**: 通过基因工程构建SERPINC1突变体重组蛋白，评估其抑制凝血酶和Xa因子的能力，发现特定突变体可增强抗凝活性，为遗传性抗凝血酶缺乏症的治疗提供新策略。

3. **文献名称**: *"Clinical Efficacy of Recombinant Antithrombin in Sepsis-Associated Coagulopathy"*

**作者**: Smith et al.

**摘要**: 临床试验表明，重组抗凝血酶III可显著降低脓毒症患者的凝血异常和器官衰竭风险，验证了其在危重症中的治疗潜力。

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如需具体文献，建议通过PubMed或Google Scholar搜索关键词 **"recombinant SERPINC1"** 或 **"recombinant antithrombin III"** 获取最新研究。

背景信息

SERPINC1. also known as antithrombin III (ATIII), is a critical serine protease inhibitor (serpin) that regulates blood coagulation by inactivating thrombin and factor Xa, key enzymes in the clotting cascade. Encoded by the SERPINC1 gene on human chromosome 1q25.1. this glycoprotein is synthesized primarily in the liver and circulates in plasma. Its activity is potentiated by heparin, enhancing anticoagulant effects by up to 1.000-fold. Deficiencies in SERPINC1—hereditary or acquired—are linked to thrombotic disorders like deep vein thrombosis and pulmonary embolism, making it a vital focus in hematology research.

Recombinant SERPINC1 protein is produced using expression systems such as mammalian cells (e.g., CHO or HEK293) to ensure proper post-translational modifications, particularly glycosylation at Asn96. Asn135. and Asn192. which influence stability and function. Unlike plasma-derived antithrombin, recombinant versions offer consistency, reduced contamination risks, and scalability. Advanced purification techniques (affinity chromatography, ion-exchange) yield high-purity products with structural and functional equivalence to native protein.

This recombinant protein serves as a therapeutic agent for congenital antithrombin deficiency, a replacement therapy during surgeries or childbirth to prevent thromboembolism. In research, it aids in studying thrombosis mechanisms, screening anticoagulant drugs, and characterizing mutations affecting heparin binding or protease inhibition. Its role as a diagnostic standard in functional assays further underscores its versatility. By bridging clinical needs and biochemical exploration, recombinant SERPINC1 remains indispensable in advancing hematologic therapeutics and understanding coagulation biology.