首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠Ⅲ型前胶原（HPCⅢ）酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的HPCⅢ含量。试剂盒检测范围为0.156 ng/mL-10 ng/mL，在肝纤维化、肺纤维化等病理过程中可作为重要生物标志物，为相关疾病机制研究提供实验依据；适用于体外实验环境下对纤维化疾病模型、药物干预效果或毒性作用研究中HPCⅢ的动态监测，为科研人员探究组织修复机制及抗纤维化治疗策略提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：procollagen type 3 ,HPC3|procollagen type III ,HPCⅢ procollagen type III ,HPCIII
缩写：HPCⅢ
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat HPCⅢ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:10 | Average % | 97 | ||||
| Range % | 91-102 | |||||
| 1:20 | Average % | 97 | ||||
| Range % | 92-104 | |||||
| 1:40 | Average % | 93 | ||||
| Range % | 90-96 | |||||
回收率：
| The recovery of rat HPCⅢ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-98 | ||||
| EDTA plasma (n=4) | 97 | 92-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 102.5892.6362.6132.426 51.9011.9671.9341.747 2.51.4551.4681.4621.275 1.251.0121.0891.0510.864 0.6250.5870.5970.5920.405 0.3120.3420.3680.3550.168 0.1560.2820.2890.2860.099 00.1880.1860.187 | ||||||
货期：3-5 working days
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