| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human BIK |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
+ +
以下是关于BIK抗体的参考文献示例(内容为模拟示例，仅供参考)：
---
1. **文献名称**: "Pro-apoptotic BIK regulates cellular apoptosis through caspase-dependent pathways"
**作者**: Li, X., Wang, H., & Chen, Y.
**摘要**: 本研究利用特异性BIK抗体，通过免疫印迹和免疫荧光技术，验证了BIK蛋白在多种癌细胞系中的表达及其促凋亡功能。研究发现，BIK通过激活caspase-3/9通路诱导凋亡，其抗体在检测内源性BIK水平中表现出高灵敏度和特异性。
2. **文献名称**: "Development and validation of a monoclonal BIK antibody for immunohistochemical analysis"
**作者**: Thompson, R., & Patel, S.
**摘要**: 文章描述了一种新型抗人BIK单克隆抗体的开发与验证。该抗体在石蜡包埋组织中表现出优异性能，成功应用于乳腺癌样本的免疫组化分析，证实BIK低表达与患者预后不良相关。
3. **文献名称**: "BIK-mediated autophagy suppression enhances chemosensitivity in colorectal cancer"
**作者**: Gupta, M., et al.
**摘要**: 研究通过BIK抗体检测发现，化疗药物上调BIK蛋白并抑制自噬，从而增强结直肠癌细胞对治疗的敏感性。实验表明BIK抗体是研究凋亡-自噬交叉调控的关键工具。
4. **文献名称**: "Functional characterization of BIK isoforms using isoform-specific antibodies"
**作者**: Zhang, L., et al.
**摘要**: 本研究开发了针对不同BIK异构体的特异性抗体，揭示不同异构体在凋亡信号中的差异功能，为理解BIK在疾病中的复杂作用提供了新工具。
---
*注：以上文献信息为示例性质，实际引用时请以真实出版物为准。如需查找真实文献，建议通过PubMed或Google Scholar搜索关键词如“BIK antibody”、“BIK apoptosis”等。*
The BIK (Bcl-2-interacting killer) protein is a pro-apoptotic member of the Bcl-2 family, which plays a critical role in regulating mitochondrial-dependent programmed cell death. Characterized by its BH3 domain, BIK promotes apoptosis by neutralizing anti-apoptotic Bcl-2 family proteins like Bcl-2 and Bcl-xL, thereby activating downstream effectors such as Bax and Bak. Dysregulation of BIK expression has been implicated in various cancers, neurodegenerative diseases, and autoimmune disorders, highlighting its importance in cellular homeostasis.
BIK antibodies are essential tools for studying its expression, localization, and interactions in both physiological and pathological contexts. These antibodies enable detection via techniques like Western blotting, immunohistochemistry, and immunofluorescence, aiding research into apoptotic signaling pathways. Additionally, they are used to explore BIK's dual roles in cancer—acting as a tumor suppressor in some contexts while contributing to chemoresistance in others—and its potential as a therapeutic target.
Recent studies also investigate BIK's involvement in non-apoptotic processes, including autophagy and endoplasmic reticulum stress. The development of specific, high-affinity BIK antibodies remains crucial for advancing mechanistic insights and translational applications in diseases linked to apoptotic dysregulation.
×
