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产品描述：人血红素氧合酶1(HO-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HMOX1含量。HMOX1即血红素氧合酶 1，可催化血红素分解。在生理状态下，其表达水平较低，但在氧化应激等多种刺激下表达上调。研究发现其与抗氧化、抗炎等机制相关，参与疾病发生发展过程，是疾病治疗潜在靶点。试剂盒检测范围为15.6 ng/mL-1000 ng/mL，可满足基础科研中HO - 1表达量分析需求，适用于探究其在神经退行性疾病、动脉粥样硬化、糖尿病并发症等领域的调控机制，或用于评估药物干预对氧化应激通路的调节作用。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：32 kD ELISA Kit; bK286B10 ELISA Kit; D8Wsu38e ELISA Kit; heat shock protein 32 kD ELISA Kit; heat shock protein 32kD ELISA Kit; Heat shock protein ELISA Kit; Heme oxygenase (decycling) 1 ELISA Kit; Heme oxygenase 1 ELISA Kit; Hemox ELISA Kit; HMOX 1 ELISA Kit; Hmox ELISA Kit; Hmox1 ELISA Kit; HMOX1_HUMAN ELISA Kit; HO 1 ELISA Kit; HO ELISA Kit; HO-1 ELISA Kit; HO1 ELISA Kit; Hsp32 ELISA Kit
缩写：HMOX1
Uniprot No.：P09601
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 ng/mL-1000 ng/mL
灵敏度：3.9 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human HO-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 80-94 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 89-99 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 92-105 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 88-99 | |||||
回收率：
| The recovery of human HO-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 85-92 | ||||
| EDTA plasma (n=4) | 98 | 90-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected10002.4492.2842.3672.2635001.9671.8641.9161.8122501.2771.2331.2551.1511250.7010.6740.6880.58462.50.4300.4210.4260.32231.20.2580.2530.2560.15215.60.1870.1840.1860.08200.1040.1030.104 | ||||||
货期：3-5 working days
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