| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human RNF2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于RNF2抗体的3篇代表性文献的简要概括(注：文献为虚拟示例，实际引用需核实)：
1. **"RNF2-mediated histone H2A ubiquitination regulates transcriptional silencing in cancer"**
*作者：Wang, H. et al.*
摘要：本研究利用RNF2抗体通过染色质免疫沉淀(ChIP)和Western blot技术，揭示了RNF2作为Polycomb复合体成员，通过催化组蛋白H2A的泛素化修饰，促进肿瘤中抑癌基因的表观遗传沉默，并证实其高表达与患者预后不良相关。
2. **"RNF2 antibody-based profiling identifies its critical role in maintaining embryonic stem cell pluripotency"**
*作者：Zhang, Y. et al.*
摘要：通过免疫荧光和Co-IP实验结合RNF2特异性抗体，研究发现RNF2通过调控多能性相关基因(如Oct4、Nanog)的染色质状态维持胚胎干细胞的自我更新，敲低RNF2导致干细胞分化标志物显著上调。
3. **"RNF2 interacts with BRCA1 and promotes DNA damage repair via antibody-validated kinase signaling modulation"**
*作者：Li, X. et al.*
摘要：采用RNF2抗体进行免疫共沉淀和组织芯片(TMA)染色，发现RNF2与BRCA1蛋白相互作用，通过泛素化修饰调控ATM/ATR信号通路，影响乳腺癌细胞对DNA损伤的修复能力，为治疗耐药性提供新靶点。
*提示：实际文献检索建议通过PubMed或Web of Science以“RNF2 antibody” + “应用技术”(如ChIP、IF、WB等)为关键词筛选，并优先选择近5年高被引论文或权威期刊(如Cell、Nature子刊)发表的研究。*
The RNF2 (Ring Finger Protein 2) antibody is a critical tool for studying the role of RNF2. a key component of the Polycomb Repressive Complex 1 (PRC1), which regulates gene silencing and epigenetic modifications. RNF2. also known as RING1B, functions as an E3 ubiquitin ligase, catalyzing the monoubiquitination of histone H2A at lysine 119 (H2AK119ub). This modification is essential for maintaining transcriptional repression of developmental genes, influencing processes like cell differentiation, proliferation, and stem cell pluripotency. Dysregulation of RNF2 has been linked to cancer progression, developmental disorders, and other diseases.
RNF2 antibodies are widely used in techniques such as Western blotting, immunoprecipitation, chromatin immunoprecipitation (ChIP), and immunofluorescence to detect protein expression, localization, and interaction partners. These antibodies help researchers investigate RNF2’s involvement in chromatin remodeling, its partnership with other Polycomb group proteins, and its impact on cellular pathways. High-quality RNF2 antibodies are validated for specificity, often through knockout/knockdown controls, and are available in various host species (e.g., rabbit, mouse) with different conjugates (e.g., HRP, fluorescent tags). Their applications span cancer research, stem cell biology, and developmental studies, offering insights into therapeutic targeting of epigenetic mechanisms.
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