| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/100-1/200 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human ARHGEF1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于ARHGEF1抗体的3篇参考文献示例(内容为虚构，仅作格式参考)：
1. **文献名称**：*ARHGEF1 regulates RhoA activation in T cell migration*
**作者**：Smith J, et al.
**摘要**：本研究利用ARHGEF1特异性抗体，通过免疫共沉淀和Western blot技术，揭示了ARHGEF1在T细胞中通过激活RhoA调控细胞骨架重组的分子机制。
2. **文献名称**：*ARHGEF1 antibody-based profiling in hepatocellular carcinoma*
**作者**：Li X, et al.
**摘要**：通过免疫组化分析，发现ARHGEF1在肝癌组织中的表达显著上调，其抗体用于临床样本检测，提示其可能作为肝癌转移的潜在生物标志物。
3. **文献名称**：*ARHGEF1 interacts with Gα13 in endothelial cell signaling*
**作者**：Garcia R, et al.
**摘要**：利用ARHGEF1抗体进行蛋白质互作研究，证实其与G蛋白亚基Gα13的结合在血管内皮细胞屏障功能调控中起关键作用。
The ARHGEF1 antibody is a tool used to detect and study ARHGEF1 (Rho Guanine Nucleotide Exchange Factor 1), a protein encoded by the ARHGEF1 gene. ARHGEF1 belongs to the RhoGEF family, which regulates Rho GTPases—key molecular switches controlling cytoskeletal dynamics, cell migration, and signaling pathways. Specifically, ARHGEF1 activates RhoA by catalyzing the exchange of GDP for GTP, influencing processes like immune cell activation, vascular function, and cancer progression. It is highly expressed in hematopoietic cells and plays a role in B-cell receptor signaling and leukocyte trafficking.
Researchers use ARHGEF1 antibodies in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to investigate its expression, localization, and interactions in cellular and tissue contexts. These antibodies are critical for elucidating ARHGEF1's role in diseases, including immune disorders, cardiovascular conditions, and malignancies. For instance, studies link ARHGEF1 dysregulation to altered immune responses and cancer metastasis. Validation of ARHGEF1 antibodies typically involves knockout cell lines or siRNA-mediated knockdown to confirm specificity. Commercially available antibodies are often raised in hosts like rabbits or mice, with reactivity across human, mouse, and rat samples. Proper controls and optimization are essential to ensure accurate detection, given potential cross-reactivity with homologous proteins.
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