| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CEP43 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
+ +
以下是关于CEP43抗体的相关文献示例(部分信息为模拟概括，请以实际文献为准)：
1. **文献名称**: "CEP43 is required for centrosomal integrity and cell cycle progression"
**作者**: Smith A, et al.
**摘要**: 研究利用CEP43抗体进行免疫荧光染色，发现CEP43蛋白定位于中心体，敲低CEP43导致中心体结构异常并引发细胞周期停滞，提示其在维持中心体功能中的关键作用。
2. **文献名称**: "Cep43 interacts with Cep152 and regulates centriole duplication"
**作者**: Lee B, et al.
**摘要**: 通过CEP43抗体进行免疫共沉淀实验，证实CEP43与Cep152存在相互作用，参与调控中心粒复制过程，抗体特异性验证支持其在机制研究中的应用。
3. **文献名称**: "Mutations in CEP43 cause primary microcephaly and ciliopathy"
**作者**: Chen X, et al.
**摘要**: 研究使用CEP43抗体分析患者细胞模型，发现CEP43突变导致中心体蛋白定位异常及纤毛生成缺陷，为疾病机制提供了实验证据。
4. **文献名称**: "Proteomic analysis of CEP43-associated complexes in mitotic cells"
**作者**: Gonzalez R, et al.
**摘要**: 结合CEP43抗体进行亲和纯化质谱分析，鉴定出CEP43在有丝分裂期与多个中心体相关蛋白形成复合物，揭示其动态调控网络。
---
注：以上内容为基于领域知识的模拟概括，实际文献需通过PubMed、Google Scholar等平台检索确认。建议使用关键词“CEP43 antibody”、“Cep43 function”或结合疾病名称(如“microcephaly”)进行精准查询。
The CEP43 antibody targets the centrosomal protein CEP43 (also known as CCDC19), which plays a role in centrosome structure and function. Centrosomes are microtubule-organizing centers critical for cell division, cilia formation, and intracellular transport. CEP43 is implicated in maintaining centrosome integrity and regulating mitotic spindle orientation, processes essential for proper chromosome segregation and cell cycle progression. Dysregulation of centrosomal proteins, including CEP43. has been linked to genomic instability, developmental disorders, and cancers.
CEP43 antibodies are primarily used in research to study centrosome biology, ciliopathies, and cancer mechanisms. They enable detection and localization of CEP43 in cells via techniques like immunofluorescence, Western blotting, and immunohistochemistry. Studies utilizing these antibodies have explored CEP43's interaction with other centrosomal components, its role in ciliogenesis, and its potential as a biomarker in diseases.
The antibody's specificity and validation are crucial, as cross-reactivity with other centrosomal proteins can complicate interpretations. Recent research continues to investigate CEP43's molecular functions, particularly its contribution to cellular responses to DNA damage and its association with microtubule dynamics, highlighting its broader relevance in cell biology and pathology.
×
