| WB | 咨询技术 | Rat |
| IF | 咨询技术 | Rat |
| IHC | 咨询技术 | Rat |
| ICC | 技术咨询 | Rat |
| FCM | 咨询技术 | Rat |
| Elisa | 咨询技术 | Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Rat |
| Immunogen | Rat IgG2b |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于Rat IgG2b抗体的3篇参考文献示例(内容为虚构，仅作格式参考)：
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1. **标题**: *Structural and Functional Characterization of Rat IgG2b Monoclonal Antibodies*
**作者**: Smith A, Jones B
**摘要**: 该研究分析了Rat IgG2b抗体的结构特征，包括其重链恒定区序列及与其他IgG亚类的差异，并探讨其在补体激活和Fc受体结合中的功能特性。
2. **标题**: *Application of Rat IgG2b in Immunohistochemical Staining of Inflammatory Tissues*
**作者**: Lee C, Wang D
**摘要**: 本文评估了Rat IgG2b抗体在小鼠炎症模型组织中的特异性染色效果，证明其与巨噬细胞标记物的高亲和力结合，适用于病理学检测。
3. **标题**: *Optimizing Protein G Affinity Purification for Rat IgG2b Subclass*
**作者**: Garcia E, Müller F
**摘要**: 研究比较了不同亲和层析介质对Rat IgG2b的纯化效率，发现Protein G在特定pH条件下对该亚类的回收率显著优于Protein A。
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*注：以上文献为示例，实际引用时需通过数据库(如PubMed)检索真实文献。*
**Background of Rat IgG2b Antibody**
Rat IgG2b is a subclass of immunoglobulin G (IgG) produced by rats, part of the adaptive immune system. Like other IgG subclasses (IgG1. IgG2a, IgG2c), it consists of two heavy chains and two light chains, with the heavy chain determining the subclass. IgG2b is characterized by a distinct hinge region structure and disulfide bonding pattern, influencing its functional properties.
Functionally, rat IgG2b plays roles in pathogen neutralization, complement activation (via the classical pathway), and engagement with Fc receptors on immune cells, facilitating phagocytosis or antibody-dependent cellular cytotoxicity (ADCC). Its ability to activate complement is generally weaker compared to IgG2a but stronger than IgG1.
In research, rat IgG2b antibodies are widely used as monoclonal antibodies (mAbs) generated through hybridoma technology. They serve as detection tools in techniques like ELISA, Western blotting, immunohistochemistry (IHC), and flow cytometry. Due to their specificity, they are also employed as isotype controls to validate assay specificity by distinguishing non-specific binding from true signals.
The choice of IgG2b over other subclasses often depends on its compatibility with secondary antibodies, effector functions, or model system requirements. Its prevalence in rat-derived mAbs makes it a critical reagent in immunology, oncology, and infectious disease studies, particularly in rodent models. Understanding its properties ensures appropriate experimental design and data interpretation.
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