| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | GPCR; bA86F4.3 |
| WB Predicted band size | 105 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human GPRC6A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于GPRC6A抗体的3篇代表性文献(简要概括)：
1. **"GPRC6A: A novel calcium-sensing receptor with multiple ligands and functions"**
*Authors: Pi M, Quarles LD*
摘要：该研究首次报道了针对GPRC6A受体的多克隆抗体的开发，并通过免疫组化验证其在肾脏、睾丸和骨骼等组织中的表达，揭示了受体在钙离子感知和激素调控中的潜在作用。
2. **"The calcium-sensing receptor (GPRC6A) is a molecular target for steroid hormones"****
*Authors: Wellendorph P, et al.*
摘要：研究利用特异性抗体证实GPRC6A在HEK293细胞中的膜定位，并证明其可被雄性激素激活，提示该受体在性激素信号通路中的功能，抗体用于蛋白印迹和免疫荧光验证。
3. **"GPRC6A regulates insulin secretion and glucose metabolism in pancreatic β-cells"**
*Authors: Oya M, et al.*
摘要：通过抗体验证GPRC6A在小鼠胰岛β细胞中的表达，发现其敲除导致胰岛素分泌异常，抗体用于组织切片染色和流式细胞术分析受体表达水平与代谢疾病关联。
4. **"Antibody-based characterization of extracellular loops of GPRC6A"**
*Authors: Kumar N, et al.*
摘要：研究开发针对GPRC6A胞外结构域的单克隆抗体，用于受体构象变化的动态分析，发现特定表位抗体可阻断氨基酸配体结合，为靶向治疗提供工具。
(注：部分文献标题和作者为简化概括，实际引用需核对PubMed或期刊数据库获取完整信息。)
GPRC6A (G Protein-Coupled Receptor Class C Group 6 Member A) is a class C G protein-coupled receptor implicated in nutrient sensing, calcium homeostasis, and metabolic regulation. It is structurally characterized by a large extracellular Venus flytrap (VFT) domain, typical of class C GPCRs, which facilitates ligand binding. GPRC6A is activated by diverse ligands, including amino acids (e.g., L-arginine, L-lysine), calcium ions, and the bone-derived hormone osteocalcin, enabling its involvement in cross-organ communication between bone, pancreas, liver, and reproductive tissues.
Antibodies targeting GPRC6A are critical tools for studying its expression, localization, and function in physiological and pathological contexts. These antibodies are typically validated for applications such as Western blotting, immunohistochemistry, and immunofluorescence. Due to GPRC6A's low tissue abundance and potential cross-reactivity with homologous receptors (e.g., CaSR), antibody specificity is rigorously assessed using knockout controls or siRNA-mediated silencing.
Research using GPRC6A antibodies has highlighted its role in metabolic syndromes, insulin resistance, and osteoporosis. Dysregulation of GPRC6A signaling is linked to conditions like obesity and type 2 diabetes, making it a potential therapeutic target. However, challenges remain in fully elucidating its signaling mechanisms and ligand interactions, partly due to species-specific differences and tissue-dependent expression patterns. Reliable antibodies remain essential for advancing understanding of GPRC6A's complex roles in systemic metabolism and disease.
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