| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | PR48; PR70; NYREN8; PPP2R3L; PPP2R3LY |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human PPP2R3B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PPP2R3B抗体的3篇参考文献示例(注：文献为假设性概括，实际引用需根据真实研究调整)：
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1. **文献名称**：*"Development of a High-Specificity Monoclonal Antibody for PPP2R3B and Its Application in Cancer Biomarker Screening"*
**作者**：Zhang Y, Li W, et al.
**摘要**：该研究报道了一种针对PPP2R3B蛋白的单克隆抗体的开发过程，通过免疫印迹(Western blot)和免疫组织化学(IHC)验证其特异性。该抗体被用于检测多种癌症组织中PPP2R3B的表达水平，发现其在乳腺癌中显著下调，提示其潜在的肿瘤抑制作用。
2. **文献名称**：*"PPP2R3B Suppresses Tumor Progression via AKT/mTOR Pathway Regulation in Colorectal Cancer"*
**作者**：Wang R, Chen T, et al.
**摘要**：研究利用PPP2R3B抗体进行功能分析，发现PPP2R3B通过抑制AKT/mTOR信号通路抑制结直肠癌细胞增殖。实验表明，PPP2R3B低表达与患者预后不良相关，抗体检测结果支持其作为预后标志物的可能性。
3. **文献名称**：*"Subcellular Localization of PPP2R3B in Neuronal Cells Using a Novel Polyclonal Antibody"*
**作者**：Kim S, Park J, et al.
**摘要**：研究团队开发了一种兔源多克隆抗体，用于PPP2R3B的亚细胞定位分析。通过免疫荧光和共聚焦显微镜技术，发现PPP2R3B主要定位于神经元胞浆，并参与突触可塑性调节，为神经退行性疾病研究提供了工具。
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**备注**：若需真实文献，建议通过PubMed或Google Scholar以“PPP2R3B antibody”或“PPP2R3B expression”为关键词检索，并筛选涉及抗体开发、验证或应用的论文。
The PPP2R3B antibody is a crucial tool for studying the regulatory subunit B'' beta (PPP2R3B) of protein phosphatase 2A (PP2A), a major serine/threonine phosphatase involved in diverse cellular processes, including cell cycle regulation, signal transduction, and apoptosis. PP2A holoenzymes consist of a catalytic subunit (C), a structural subunit (A), and a variable regulatory subunit (B), with PPP2R3B belonging to the B''/PR72 family. This subunit directs PP2A’s substrate specificity, localization, and activity, playing roles in Wnt, MAPK, and Akt signaling pathways.
Antibodies targeting PPP2R3B are used to investigate its expression, post-translational modifications, and interactions in tissues or cell lines. They enable detection via techniques like Western blotting, immunofluorescence, and immunohistochemistry. Research has linked PPP2R3B dysregulation to diseases such as cancer and neurodegenerative disorders, where altered PP2A activity contributes to pathogenic signaling. For example, PPP2R3B may act as a tumor suppressor by modulating PP2A-mediated dephosphorylation of oncogenic kinases.
These antibodies are typically raised against specific epitopes, often validated using knockout controls or siRNA-mediated silencing to ensure specificity. Recent studies also explore PPP2R3B’s structural domains, particularly its conserved regions critical for binding PP2A core enzymes and other regulatory proteins. Understanding PPP2R3B’s role through antibody-based assays aids in deciphering PP2A’s complex regulatory networks and its implications in disease mechanisms.
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