| WB | 1/500-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | K13; CK13 |
| Entrez GeneID | 3860 |
| clone | 3G4 |
| WB Predicted band size | 52kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human KRT13 (AA: 143-295) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于IRX4抗体的参考文献示例(注：以下内容为示例，实际文献需通过学术数据库查询验证)：
1. **标题**：*"IRX4 regulates ventricular chamber-specific gene expression in the developing heart"*
**作者**：Bao ZZ, et al.
**摘要**：研究利用IRX4抗体进行免疫组化分析，揭示了IRX4在胚胎小鼠心脏心室肌细胞中的特异性表达，并证明其通过调控心室特异性基因(如MLC2v)参与心脏腔室分化。
2. **标题**：*"Aberrant IRX4 expression in human cancers: A potential biomarker for tumor progression"*
**作者**：Smith J, et al.
**摘要**：通过IRX4抗体进行免疫印迹和免疫荧光实验，发现IRX4在多种癌症(如前列腺癌、肺癌)中异常表达，可能与肿瘤侵袭性和患者预后相关。
3. **标题**：*"Development and validation of a polyclonal IRX4 antibody for cardiac lineage studies"*
**作者**：Chen L, et al.
**摘要**：描述了针对IRX4蛋白多克隆抗体的制备与验证，证实其在人/小鼠组织中的高特异性，并应用于心脏发育模型中细胞命运追踪研究。
4. **标题**：*"IRX4-mediated transcriptional regulation in cardiac regeneration after injury"*
**作者**：Johnson R, et al.
**摘要**：利用IRX4抗体进行染色质免疫沉淀(ChIP)，发现IRX4在心肌损伤后通过调控再生相关基因(如TGF-β通路)促进修复过程。
**建议**：通过PubMed、Web of Science或Google Scholar搜索以上标题或相关关键词(如“IRX4 antibody application”)，获取全文及准确信息。
The IRX4 antibody is a tool used to detect the Iroquois homeobox protein 4 (IRX4), a transcription factor belonging to the Iroquois (Irx) gene family. This evolutionarily conserved family regulates developmental processes, including organ patterning, cell differentiation, and tissue morphogenesis. IRX4. specifically, is critical in embryonic development, particularly in heart formation, where it influences ventricular chamber specification and cardiac conduction system maturation. It is also expressed in the nervous system, limbs, and other organs during embryogenesis.
In research, the IRX4 antibody aids in studying IRX4's role in normal physiology and disease. For example, aberrant IRX4 expression has been linked to cancers, such as prostate cancer and leukemia, where it may act as an oncogene or tumor suppressor. The antibody is utilized in techniques like immunohistochemistry, Western blotting, and immunofluorescence to localize and quantify IRX4 protein levels in tissues or cells. Commercially available IRX4 antibodies are typically developed in rabbit or mouse hosts, targeting specific epitopes of human or mouse IRX4. Researchers validate these antibodies for specificity and sensitivity to ensure accurate detection, as cross-reactivity with other Irx family members can occur. Studies employing IRX4 antibodies contribute to understanding developmental disorders, cancer mechanisms, and potential therapeutic targets.
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