| WB | 1/300-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | IRX-1; IRXB1 |
| WB Predicted band size | 52 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human IRX3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于IRX3抗体的参考文献示例(注：以下内容为模拟生成，实际文献需通过学术数据库验证)：
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1. **文献名称**：*IRX3 Controls Obesity by Regulating Neuronal Energy Homeostasis*
**作者**：Smas C. et al.
**摘要**：研究揭示了IRX3转录因子在下丘脑神经元中对能量平衡的调控作用。通过IRX3抗体染色，发现其在小鼠下丘脑特定神经元中高表达，并证明IRX3缺失会导致代谢异常和肥胖表型。
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2. **文献名称**：*Developmental Role of IRX3 in Neural Crest Cell Differentiation*
**作者**：Kim D.W. et al.
**摘要**：利用IRX3抗体进行免疫组化分析，发现IRX3在胚胎神经嵴细胞中特异性表达，并通过调控Wnt信号通路影响细胞分化和迁移，为神经发育疾病提供了潜在机制。
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3. **文献名称**：*IRX3 as a Novel Biomarker in Colorectal Cancer Progression*
**作者**：Zhang Y. et al.
**摘要**：通过IRX3抗体检测人类结直肠癌组织，发现IRX3表达水平与肿瘤侵袭性呈正相关，提示其可能作为癌症预后的分子标志物和治疗靶点。
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4. **文献名称**：*Functional Analysis of IRX3 in Pancreatic Beta Cells*
**作者**：Rui J. et al.
**摘要**：研究利用IRX3抗体结合ChIP-seq技术，揭示了IRX3在胰岛β细胞中调控胰岛素分泌相关基因的机制，为糖尿病研究提供了新方向。
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建议通过PubMed或Google Scholar搜索关键词“IRX3 antibody”或“IRX3 function”以获取真实文献。
The IRX3 antibody is a research tool targeting the Iroquois homeobox protein 3 (IRX3), a transcription factor encoded by the *IRX3* gene. IRX3 belongs to the Iroquois (Irx) gene family, which plays critical roles in embryonic development, particularly in patterning the nervous system, limbs, and heart. IRX3 regulates gene expression during cell differentiation and tissue morphogenesis, with studies linking it to neurodevelopment, metabolic regulation, and body weight control. Notably, genome-wide association studies (GWAS) identified *IRX3* as a candidate obesity-associated gene, suggesting its influence on energy homeostasis and adipogenesis.
IRX3 antibodies are primarily used in experimental settings to detect and localize IRX3 protein expression via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These antibodies aid in studying IRX3's spatial-temporal expression patterns, interactions with signaling pathways (e.g., Wnt, Shh), and its role in diseases such as obesity, diabetes, and cancer. Commercial IRX3 antibodies are typically raised in rabbits or mice, validated for specificity in human or model organisms (e.g., mouse, zebrafish). Challenges include cross-reactivity with homologous family members (e.g., IRX5. IRX6), necessitating careful validation. Research using IRX3 antibodies continues to uncover its regulatory networks and therapeutic potential in metabolic disorders.
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