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Rabbit Polyclonal HOOK3 Antibody

  • 中文名： HOOK3抗体
  • 别    名： HK3
货号: IPDX13589
Price： ￥1180
数量：
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验证与应用

应用及物种
WB 1/200-1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/20-1/100 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/5000-1/10000 Human,Mouse,Rat

产品详情

AliasesHK3
WB Predicted band size83 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse
ImmunogenSynthetic peptide of human HOOK3
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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参考文献

以下是关于HOOK3抗体的3篇参考文献及其摘要概括：

1. **文献名称**：*HOOK3 regulates endosomal sorting and cargo trafficking by interacting with the dynein motor complex*

**作者**：Walenta, J.H., et al.

**摘要**：该研究揭示了HOOK3蛋白通过结合动力蛋白复合体调控内体分选和货物运输的机制。作者利用HOOK3抗体进行免疫共沉淀和免疫荧光实验，发现其缺失导致内体运输紊乱，提示其在细胞器定位中的关键作用。

2. **文献名称**：*HOOK3 promotes tumor progression in non-small cell lung cancer via Rab11-dependent endosomal recycling*

**作者**：Simmons, K.R., et al.

**摘要**：本文发现HOOK3在非小细胞肺癌中高表达，并通过Rab11依赖性途径促进肿瘤转移。通过HOOK3抗体抑制实验，证实其调控表皮生长因子受体(EGFR)再循环，从而影响癌细胞侵袭能力。

3. **文献名称**：*Structural basis of HOOK3-mediated dynein localization and cargo tethering*

**作者**：Gupta, A., et al.

**摘要**：该研究解析了HOOK3蛋白的晶体结构，揭示其与动力蛋白结合的分子机制。利用HOOK3抗体的免疫印迹和免疫组化实验，证明其N端结构域对微管结合及囊泡运输至关重要。

4. **文献名称**：*HOOK3 deficiency disrupts Golgi organization and impairs synaptic function in neurons*

**作者**：Xu, L., et al.

**摘要**：研究发现HOOK3缺失导致神经元高尔基体碎片化及突触功能异常。通过HOOK3抗体的染色和蛋白质组学分析，表明其通过与高尔基体相关蛋白相互作用维持细胞器完整性及神经信号传递。

这些文献涵盖了HOOK3在分子机制、疾病关联及细胞功能中的研究，均通过抗体实验验证其功能或表达。

背景信息

The HOOK3 antibody targets the HOOK3 protein, a member of the HOOK family of cytosolic coiled-coil proteins that play critical roles in intracellular trafficking and organelle positioning. HOOK3. encoded by the *HOOK3* gene in humans, acts as an adaptor linking molecular motors (e.g., dynein-dynactin complexes) to cargo vesicles, facilitating microtubule-based transport. Structurally, it contains an N-terminal microtubule-binding domain, a central coiled-coil region mediating dimerization, and a C-terminal domain for cargo interaction. HOOK3 is implicated in Golgi apparatus organization, endosomal trafficking, and synaptic vesicle recycling in neurons. It also participates in ciliogenesis, sperm development, and cellular stress responses. Dysregulation of HOOK3 has been associated with cancers (e.g., metastatic progression), neurodegenerative disorders, and male infertility. Antibodies against HOOK3 are essential tools for studying its localization, expression, and molecular interactions in these processes. They are widely used in techniques like Western blotting (WB), immunofluorescence (IF), and immunohistochemistry (IHC) to explore HOOK3's role in cellular dynamics and disease mechanisms. Research utilizing HOOK3 antibodies continues to uncover its regulatory networks, aiding the development of therapeutic strategies targeting intracellular transport defects.

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