首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠肝细胞生长因子(HGF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HGF含量。HGF即肝细胞生长因子，是一种多功能细胞因子。其可与受体c-Met结合激活下游多条信号通路。在生理上参与胚胎发育、组织修复等过程；在病理方面，和肿瘤的发生、发展、转移等密切相关，因此成为肿瘤等疾病治疗研究的重要靶点。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于基础科研中HGF表达水平监测，如肝脏再生机制研究、药物干预效果评价或病理模型中的分子动态追踪，为炎症反应、纤维化进程及肿瘤相关研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：HgfHepatocyte growth factor ELISA Kit; Hepatopoietin-A ELISA Kit; Scatter factor ELISA Kit; SF) [Cleaved into: Hepatocyte growth factor alpha chain; Hepatocyte growth factor beta chain] ELISA Kit
缩写：HGF
Uniprot No.：P17945
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat HGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 93-103 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 91-97 | |||||
| 1:4 | Average % | 108 | ||||
| Range % | 98-112 | |||||
| 1:8 | Average % | 87 | ||||
| Range % | 80-92 | |||||
回收率：
| The recovery of rat HGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 86-94 | ||||
| EDTA plasma (n=4) | 95 | 91-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.1852.2842.2352.06151.7031.8021.7531.5792.51.2761.2951.2861.1121.250.9030.8940.8990.7250.6250.6500.6410.6460.4720.3120.4110.4020.4070.2330.1560.3190.3120.3160.14200.1730.1740.174 | ||||||
货期：3-5 working days
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