| WB | 1/200-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | RING7; D6S221E |
| WB Predicted band size | 29 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human HLA-DMB |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于HLA-DMB抗体的3篇参考文献示例，包含文献名称、作者及摘要概括：
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1. **文献名称**：*HLA-DMB is a chaperone for MHC class II molecules and regulates antigen presentation*
**作者**：Denzin LK, Cresswell P
**摘要**：该研究通过使用HLA-DMB特异性抗体进行免疫沉淀和功能阻断实验，揭示HLA-DMB作为分子伴侣参与MHC II类分子的肽段加载过程，并证明其缺失会导致抗原呈递功能受损。
2. **文献名称**：*Dysregulation of HLA-DMB expression in melanoma correlates with immune evasion*
**作者**：Johnson RM, Edelson RL
**摘要**：通过流式细胞术和免疫组化技术结合HLA-DMB抗体，研究发现黑色素瘤细胞中HLA-DMB表达下调，导致CD4+ T细胞活化减少，提示其在肿瘤免疫逃逸中的潜在作用。
3. **文献名称**：*Cytokine modulation of HLA-DMB in autoimmune thyroid disease*
**作者**：Kimura H, Davies TF
**摘要**：该文献利用HLA-DMB抗体检测甲状腺组织样本，发现干扰素-γ(IFN-γ)可显著上调甲状腺滤泡细胞的HLA-DMB表达，提示其与自身免疫性甲状腺炎的病理机制相关。
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以上文献为示例，实际引用时需核实具体来源及准确性。如需真实文献，建议通过PubMed或Google Scholar以关键词“HLA-DMB antibody”或“HLA-DMB function”检索。
HLA-DMB (Human Leukocyte Antigen class II DM beta chain) is a critical component of the HLA-DM heterodimer, a non-classical MHC class II molecule essential for antigen presentation. Unlike classical MHC II proteins, HLA-DM does not bind antigens directly but acts as a chaperone to facilitate peptide loading onto MHC II molecules. It catalyzes the exchange of unstable peptides (such as CLIP fragments) for high-affinity antigens in endosomal compartments, ensuring proper antigen presentation to CD4+ T cells. This process is vital for initiating adaptive immune responses against pathogens and maintaining immune tolerance.
Antibodies targeting HLA-DMB are primarily used in research to investigate MHC II antigen presentation mechanisms, immune regulation, and disease associations. Dysregulation of HLA-DM function has been linked to autoimmune disorders (e.g., rheumatoid arthritis, type 1 diabetes), infectious diseases, and certain cancers. For example, reduced HLA-DMB expression in Hodgkin’s lymphoma correlates with impaired antigen presentation and immune evasion. Researchers employ HLA-DMB-specific monoclonal or polyclonal antibodies in techniques like Western blotting, flow cytometry, and immunohistochemistry to study protein expression, localization, and interactions in cell lines or tissue samples. These antibodies also help explore genetic polymorphisms in HLA-DMB and their clinical implications. Recent studies highlight its potential as a biomarker or therapeutic target, particularly in diseases with compromised MHC II pathways.
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