| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | BDR2; DYT2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human HPCA |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于HPCA抗体的参考文献示例(注：文献信息为模拟生成，仅供参考)：
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1. **文献名称**：*Hippocalcin, a Calcium-Binding Protein Enriched in Hippocampal Neurons*
**作者**：Kobayashi M, Takamatsu K, Saitoh S
**摘要**：研究首次克隆并鉴定了HPCA(Hippocalcin)的分子特性，证实其在海马神经元中高表达。通过特异性抗体的开发，揭示了HPCA在钙离子依赖性信号通路中的作用，可能参与调节神经元兴奋性与突触可塑性。
2. **文献名称**：*Immunohistochemical Localization of Hippocalcin in Rat Brain Using a Novel Monoclonal Antibody*
**作者**：Terao M, Nakamura K, Tanaka T
**摘要**：报道了一种新型HPCA单克隆抗体的制备，并利用免疫组化技术系统分析了HPCA在大鼠脑区的分布。结果显示其在海马CA1区、皮层及小脑浦肯野细胞中显著表达，提示其功能多样性。
3. **文献名称**：*Hippocalcin Deficiency Impairs Synaptic Plasticity and Spatial Memory in Mice*
**作者**：Wang Y, Li X, Zhang H
**摘要**：通过HPCA基因敲除小鼠模型，结合抗体标记技术，发现HPCA缺失导致长时程增强(LTP)受损及空间记忆障碍，证明其在突触功能与学习记忆中的关键作用。
4. **文献名称**：*Altered Hippocalcin Expression in Alzheimer's Disease Postmortem Brain Tissue*
**作者**：Smith J, Doe R, Johnson A
**摘要**：利用HPCA抗体对阿尔茨海默病患者脑组织进行分析，发现海马区HPCA表达显著降低，且与β-淀粉样蛋白沉积呈负相关，提示其可能参与神经退行性病变机制。
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建议通过PubMed、Google Scholar等平台检索真实文献，以“Hippocalcin antibody”“HPCA neuronal function”等关键词获取最新研究。
**Background of HPCA Antibody**
Hippocalcin (HPCA), a calcium-sensing protein predominantly expressed in the brain, belongs to the neuronal calcium sensor (NCS) family. It plays a critical role in regulating intracellular calcium signaling, synaptic plasticity, and neuronal excitability. HPCA is particularly abundant in hippocampal neurons, where it modulates calcium-dependent processes linked to learning, memory, and neuroprotection. Dysregulation of HPCA has been implicated in neurological disorders, including epilepsy, Alzheimer’s disease, and ischemic brain injury.
HPCA antibodies are essential tools for investigating its expression, localization, and function in both physiological and pathological contexts. These antibodies are typically developed using immunogenic peptides derived from conserved regions of the HPCA protein, enabling specificity in techniques like Western blotting, immunohistochemistry, and immunofluorescence. Researchers utilize HPCA antibodies to study its interaction with downstream targets (e.g., ion channels, kinases) and its role in calcium buffering or apoptosis. Recent studies also explore HPCA’s potential as a biomarker for neurodegenerative diseases.
The development and validation of HPCA antibodies have advanced understanding of calcium-mediated signaling pathways in the brain, offering insights into therapeutic strategies for neurological conditions. Commercial HPCA antibodies are often validated across species, including human, mouse, and rat models, ensuring broad applicability in neuroscience research.
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