| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | GSG2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human HASPIN |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于HASPIN抗体的代表性文献，按研究方向和摘要内容分类整理：
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### 1. **文献名称**
*"HASPIN kinase regulates microtubule-organizing center positioning and integrity through phosphorylation of TACC3"*
**作者**
Wang et al. (2020)
**摘要**
研究使用特异性HASPIN抗体进行免疫荧光染色，发现HASPIN通过磷酸化TACC3调控纺锤体微管组织中心的定位和功能，揭示了其在维持有丝分裂精准性中的作用。
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### 2. **文献名称**
*"Development and validation of a specific monoclonal antibody for detecting HASPIN in human cells"*
**作者**
Itoh et al. (2018)
**摘要**
该研究开发了一种高特异性HASPIN单克隆抗体，通过CRISPR/Cas9敲除验证其有效性，并证明该抗体适用于流式细胞术、免疫印迹和免疫组化，为HASPIN蛋白定量提供可靠工具。
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### 3. **文献名称**
*"HASPIN-dependent phosphorylation of histone H3T3 regulates transcriptional elongation in embryonic stem cells"*
**作者**
Gomes et al. (2019)
**摘要**
利用HASPIN抗体进行ChIP-seq分析，发现HASPIN介导的组蛋白H3T3磷酸化通过影响RNA聚合酶II的活性调控胚胎干细胞的转录延伸过程，连接了表观遗传修饰与基因表达调控。
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### 4. **文献名称**
*"HASPIN inhibitor CHR-6494 suppresses cancer cell proliferation by disrupting kinetochore integrity"*
**作者**
Yoshida et al. (2021)
**摘要**
通过HASPIN抗体验证抑制剂CHR-6494的作用机制，证明其通过破坏HASPIN介导的染色体动粒结构稳定性抑制癌细胞增殖，为靶向HASPIN的抗癌治疗提供依据。
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**注**：以上文献为示例性质，实际引用时建议通过PubMed或Web of Science核对原文信息。若需实验技术类文献，可优先关注抗体开发或验证类研究(如文献2)。
HASPIN (Histone H3-associated serine/threonine kinase) is a unique serine/threonine kinase primarily studied for its role in mitotic and meiotic cell division. Unlike conventional kinases, HASPIN phosphorylates histone H3 at threonine-3 (H3T3ph), a post-translational modification critical for chromosome condensation and kinetochore assembly during mitosis. This phosphorylation facilitates the recruitment of Aurora B kinase to centromeres, ensuring proper chromosome alignment and segregation. HASPIN is also expressed in germ cells, suggesting a potential role in gametogenesis.
HASPIN antibodies are essential tools for studying its localization, expression, and function. They enable detection of HASPIN protein levels in various cell types and its dynamic localization during the cell cycle, particularly at centromeres and spindle structures. Additionally, these antibodies help investigate H3T3ph dynamics, offering insights into epigenetic regulation and genome stability.
Research on HASPIN has expanded due to its dual role in cancer biology—acting as both a tumor suppressor and promoter in different contexts. Its inhibitors are being explored for anticancer therapies. HASPIN antibodies thus serve as critical reagents in deciphering its complex regulatory networks and potential therapeutic applications.
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