首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠肝素结合EGF样生长因子(HBEGF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HBEGF含量。HBEGF是一种重要的靶点。它是表皮生长因子家族成员，在细胞生长、增殖和迁移等过程中发挥作用。研究聚焦于其在肿瘤发生、发展中的机制，比如促进肿瘤细胞生长、侵袭和转移等，有望为癌症等疾病治疗提供新方向。试剂盒检测范围为6.25 pg/mL-400 pg/mL，该产品适用于科研领域研究HBEGF在肝损伤、心血管疾病或肿瘤模型中的表达调控机制，例如通过检测肝纤维化模型中组织匀浆HBEGF水平变化，或分析药物干预后血浆因子动态变化，为探索HBEGF相关信号通路及疾病机理提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Hbegf; Dtr; Hegfl; Proheparin-binding EGF-like growth factor
缩写：HBEGF
Uniprot No.：Q06175
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat HBEGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 104 | |||||
| Range % | 91-108 | ||||||
| 1:2 | Average % | 100 | |||||
| Range % | 91-105 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 87-99 | ||||||
| 1:8 | Average % | 98 | |||||
| Range % | 90-103 | ||||||
回收率：
| The recovery of rat HBEGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 89 | 81-95 | |||||
| EDTA plasma (n=4) | 102 | 95-107 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected4002.7272.5862.6572.5372002.2292.1792.2042.0841001.4521.4281.4401.320500.7390.7270.7330.613250.4230.4110.4170.29712.50.2680.2610.2650.1456.250.1820.1750.1790.05900.1180.1220.120 | |||||||
货期：3-5 working days
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