| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPRD1A |
| Uniprot No | Q96P16 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-312aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSEFMSAFS EAALEKKLSE LSNSQQSVQT LSLWLIHHRK HSRPIVTVWE RELRKAKPNR KLTFLYLAND VIQNSKRKGP EFTKDFAPVI VEAFKHVSSE TDESCKKHLG RVLSIWEERS VYENDVLEQL KQALYGDKKP RKRTYEQIKV DENENCSSLG SPSEPPQTLD LVRALQDLEN AASGDAAVHQ RIASLPVEVQ EVSLLDKITD KESGERLSKM VEDACMLLAD YNGRLAAEID DRKQLTRMLA DFLRCQKEAL AEKEHKLEEY KRKLARVSLV RKELRSRIQS LPDLSRLPNV TGSHMHLPFA GDIYSED |
| 预测分子量 | 38 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPRD1A重组蛋白的示例参考文献(仅供参考，非真实文献)：
1. **文献名称**: "RPRD1A interacts with RNA polymerase II to regulate transcriptional termination"
**作者**: Davidson G., et al.
**摘要**: 研究通过重组RPRD1A蛋白的体外实验，揭示了其与RNA聚合酶II的CTD结构域相互作用，调控转录终止过程的分子机制。
2. **文献名称**: "Structural and functional analysis of RPRD1A in cell cycle regulation"
**作者**: Hirose Y., et al.
**摘要**: 利用重组RPRD1A蛋白的晶体结构解析，阐明其通过结合Cyclin K/P-TEFb复合物调控细胞周期进程的功能，并影响RNA聚合酶II的磷酸化状态。
3. **文献名称**: "RPRD1A suppresses tumorigenesis via modulation of DNA damage response"
**作者**: Zhang L., et al.
**摘要**: 通过重组蛋白表达实验，证实RPRD1A参与DNA损伤应答通路，与ATM激酶相互作用并调控下游修复因子的活性，抑制肿瘤发生。
4. **文献名称**: "RPRD1A dysfunction promotes cancer metastasis through aberrant Wnt signaling"
**作者**: Lee S., et al.
**摘要**: 研究利用重组RPRD1A蛋白的体外功能恢复实验，表明其缺失导致β-catenin异常积累，激活Wnt信号通路并促进癌细胞侵袭转移。
(注：以上为模拟文献，实际研究中建议通过PubMed、Web of Science等平台检索具体文献。)
**Background of RPRD1A Recombinant Protein**
RPRD1A (Regulation of Nuclear Pre-mRNA Domain-Containing Protein 1A), also known as C7orf28A or CRSP7. is a conserved eukaryotic protein involved in transcriptional regulation and RNA processing. It contains a CID (CTD-interacting domain) that enables interaction with the C-terminal domain (CTD) of RNA polymerase II (Pol II), a critical component of the transcription machinery. This interaction links RPRD1A to transcription elongation, termination, and co-transcriptional processes like mRNA capping and splicing.
RPRD1A functions as a scaffold protein, coordinating interactions between Pol II and regulatory complexes, including the Integrator complex and the cyclin-dependent kinase (CDK)-activated phosphatase (SCP). Studies suggest it plays a role in maintaining transcription fidelity, cell cycle progression, and DNA damage response. For instance, RPRD1A associates with ATM/ATR kinases to modulate checkpoint signaling upon genotoxic stress. Dysregulation of RPRD1A has been implicated in cancers, neurodegenerative disorders, and developmental defects, highlighting its biological and clinical relevance.
Recombinant RPRD1A protein is engineered for in vitro studies to dissect its molecular mechanisms. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), it retains functional domains for binding nucleic acids or partner proteins. Researchers utilize it in pull-down assays, structural studies (e.g., crystallography), and enzymatic activity assays to explore its role in transcription-coupled processes. Additionally, recombinant RPRD1A serves as an antigen for antibody generation, aiding in cellular localization and expression profiling.
Overall, RPRD1A recombinant protein is a vital tool for unraveling the multifaceted roles of RPRD1A in gene expression and disease, offering insights into potential therapeutic targets.
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